Whole-genome bisulfite sequencing of goat skins identifies signatures associated with hair cycling.
Whole-genome bisulfite sequencing of goat skins identifies signatures associated with hair cycling.
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山羊皮的全基因组亚硫酸氢盐测序确定了与毛发循环相关的特征
DOI：
10.1186/s12864-018-5002-5
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发表时间：
2018-08-28
期刊：
影响因子：
4.4
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li C;Li Y;Zhou G;Gao Y;Ma S;Chen Y;Song J;Wang X
背景毛囊（Hair follicles，HF）在发育过程中经历生长期（anagen）、退化期（catagen）和休止期（telogen）的重复周期。这些阶段之间的转换是由多种分子信号决定的，包括DNA甲基化，它在哺乳动物细胞身份中起重要作用，并且对HF的发展至关重要。次级毛囊（SHFs）在绒山羊表现出经典的周期性毛发发育，并没有做过什么在全基因组范围内检查潜在的甲基化基因参与的毛发cyclic transition.ResultsGenome-wide的DNA甲基化模式之间的皮肤组织采样在生长期和休止期阶段的绒山羊进行了研究，使用全基因组亚硫酸氢盐测序（WGBS）。在静止期具有HF的皮肤样品中观察到甲基化状态高于生长期阶段的那些。在两组之间总共鉴定了1311个差异甲基化区域（DMR），其中包含493个完全注释的DMR相关基因（DMG）（269个Hyper-DMG和224个Hypo-DMG）。此外，观察到与毛发循环期间的免疫应答和细胞间串扰相关的DMG的功能类别的显著过度代表性。通过整合DNA甲基化和mRNA表达数据，我们发现，四个基因（FMN 1，PCOLCE，SPTLC 3，和COL 5A 1）是阐明有助于休止期到生长期transition.ConclusionOur研究的表观遗传机制的关键因素提供了系统的甲基化图谱有关的头发周期阶段（生长期与休止期）在一个单一的碱基分辨率，并揭示了特定阶段的甲基化位点在羊绒生长或静止。此外，我们确定了表观遗传调控基因，可能参与HF的发展和增长，在绒山羊，并可能在其他哺乳动物物种。
BackgroundHair follicles (HFs), upon development, undergo repetitive cycles of growth (anagen), regression (catagen), and rest (telogen). The transition between the stages is determined by multiple molecular signals, including DNA methylation, which plays important roles in mammalian cellular identity and is essential for the development of HFs. Secondary hair follicles (SHFs) in cashmere goat exhibit classic cyclic hair development, and little has been done on a genome-wide scale to examine potentially methylated genes involved in the hair cyclic transition.ResultsGenome-wide DNA methylation profiles between skin tissues sampled during the anagen and telogen stages in cashmere goats were investigated using whole-genome bisulfite sequencing (WGBS). The methylation status was observed to be higher in the skin samples with HFs in the telogen than those in the anagen stage. A total of 1311 differentially methylated regions (DMRs) were identified between the two groups, which contained 493 fully annotated DMR-related genes (DMGs) (269 Hyper- DMGs and 224 Hypo-DMGs). Furthermore, a significant over-representation of the functional categories for DMGs related to immune response and intercellular crosstalk during hair cycling was observed. By integrating DNA methylation and mRNA expression data, we revealed that four genes (FMN1,PCOLCE,SPTLC3, andCOL5A1) are crucial factors for elucidating epigenetic mechanisms contributing to the telogen-to-anagen transition.ConclusionOur study provided systematic methylome maps pertaining to the hair cycling stages (anagen vs telogen) at a single-base resolution, and revealed stage-specific methylation loci during cashmere growth or quiescence. Furthermore, we identified epigenetically regulated genes that are potentially involved in HF development and growth in cashmere goats, and likely in other mammal species.