The measurement of oxygen in vivo using EPR techniques
The measurement of oxygen in vivo using EPR techniques
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DOI：
10.1088/0031-9155/43/7/017
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发表时间：
1998-07-01
影响因子：
3.5
通讯作者：
Clarkson, RB
中科院分区：
文献类型：
--
作者：
Swartz, HM;Clarkson, RB
利用EPR测量体内pO（2）具有一些已经导致非常有用的应用的特征，并且这种方法可能具有越来越广泛和有效的用途。它是基于氧对EPR谱的影响，为定量测定pO（2）提供了一种灵敏、准确的方法。氧敏感的顺磁材料，这是非常稳定的，结合仪器的发展，发展一直是至关重要的，在体内应用这项技术。本文综述了在体EPR血氧测定的物理基础和生物学应用，特别强调了在1 GHz下使用颗粒顺磁材料的EPR光谱用于组织中pO（2）的重复性和非侵入性测量。体内EPR已经产生了一些非常有用的结果，这些结果对解决重要的生物学问题做出了重大贡献。EPR血氧饱和度的特点，这似乎是特别有用的，往往是补充现有的技术，用于测量组织中的氧气。这些特性包括能够从同一部位进行重复测量，对低氧水平具有高灵敏度，以及非侵入性选择。现有的技术对于小动物的研究特别有用，因为测量的深度不是首要问题。在较大的动物和潜在的人类受试者中，非侵入性技术似乎立即适用于研究非常接近表面（10 mm内）的现象，而侵入性技术具有一些非常有前途的用途。EPR血氧测定法的临床应用似乎特别有前途，并可能在不久的将来进行长期监测的状态和治疗周围血管疾病的反应，并优化癌症治疗，使其能够修改的基础上，在肿瘤中测量的pO（2）。
The measurement of pO(2) in vivo using EPR has some features which have already led to very useful applications and this approach is likely to have increasingly wide and effective use. It is based on the effect of oxygen on EPR spectra which provides a sensitive and accurate means to measure pO(2) quantitatively. The development of oxygen-sensitive paramagnetic materials which are very stable, combined with instrumental developments, has been crucial to the in vivo applications of this technique. The physical basis and biological applications of in vivo EPR oximetry are reviewed, with particular emphasis on the use of EPR spectroscopy at 1 GHz using particulate paramagnetic materials for the repetitive and non-invasive measurement of pO(2) in tissues. In vivo EPR has already produced some very useful results which have contributed significantly to solving important biological problems. The characteristics of EPR oximetry which appear to be especially useful are often complementary to existing techniques for measuring oxygen in tissues. These characteristics include the capability of making repeated measurements from the same site, high sensitivity to low levels of oxygen, and non-invasive options. The existing techniques are especially useful for studies in small animals, where the depth of measurements is not an overriding issue. In larger animals and potentially in human subjects, non-invasive techniques seem To be immediately applicable to study phenomena very near the surface (within 10 mm) while invasive techniques have some very promising uses. The clinical uses of EPR oximetry which seem especially promising and likely to be undertaken in the near future are long-term monitoring of the status and response to treatment of peripheral vascular disease and optimizing cancer therapy by enabling it to be modified on the basis of the pO(2) measured in the tumour.