Artifacts in Magnetic Force Microscopy of Histological sections
Artifacts in Magnetic Force Microscopy of Histological sections
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DOI：
10.1016/j.jmmm.2022.170116
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发表时间：
2022-10
影响因子：
2.7
通讯作者：
Kevin J. Walsh;Owen Shiflett;Stavan Shah;T. Renner;Nicholas Soulas;D. Scharre;Dana McTigue;G. Agarwal
中科院分区：
文献类型：
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作者：
Kevin J. Walsh;Owen Shiflett;Stavan Shah;T. Renner;Nicholas Soulas;D. Scharre;Dana McTigue;G. Agarwal
生物样品中氧化铁纳米颗粒的检测具有广泛的应用。铁可以作为铁蛋白（铁）的生理性水铁矿颗粒或作为某些神经退行性病变中的生物源性磁铁矿纳米颗粒天然存在于生物组织中。在我们以前的研究中，我们已经报道了如何磁力显微镜（MFM）可以用来映射铁沉积在组织切片中的无标记的方式。在这项研究中，为了提高MFM组织学分析的效率，我们探讨了提高扫描速率的效果。我们的研究结果表明，如何MFM图像的组织切片可以被污染的文物，由于地形串扰，特别是在较高的扫描速率。在啮齿动物脾脏以及从阿尔茨海默病患者获得的脑组织切片中观察到这些伪影。我们的研究结果表明，如何地形串扰可以使它具有挑战性的明确检测组织铁通过MFM。多模式办法有助于克服其中一些限制。
Detection of iron-oxide nanoparticles in biological samples has widespread applications. Iron can be naturally present in biological tissues as physiological ferrihydrite particles of ferritin(iron) or as biogenic magnetite nanoparticles in certain neurodegenerative pathologies. In our previous studies, we had reported how magnetic force microscopy (MFM) can be used to map iron deposits in tissue sections in a label-free manner. In this study, to improve the efficiency of MFM for histological analysis, we explored the effect of increasing scan rate. Our results show how MFM images of tissue sections can be contaminated with artifacts due to topographical cross-talk, especially at higher scan rates. These artifacts were observed in rodent spleen as well as in sections of brain tissue obtained from patients with Alzheimer’s Disease. Our results show how topographical cross talk can make it challenging to unambiguously detect histological iron via MFM. Multimodal approaches can help overcome some of these limitations.