Specific antibodies to mouse Sca-1-(Ly-6A/E) or Thy-1-positive haematopoietic progenitor cells induce formation of nitric oxide which inhibits subsequent colony formation
Specific antibodies to mouse Sca-1-(Ly-6A/E) or Thy-1-positive haematopoietic progenitor cells induce formation of nitric oxide which inhibits subsequent colony formation
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DOI：
10.1111/j.1600-0609.2004.00322.x
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发表时间：
2004-12-01
影响因子：
3.1
通讯作者：
Lovhaug, D
中科院分区：
文献类型：
--
作者：
Boyum, A;Fjerdingstad, HB;Lovhaug, D
将小鼠骨髓细胞暴露于特异性单克隆抗体，以便可以用磁珠去除谱系阳性（Lin（+））细胞。将Lin（-）细胞与Sca-1或CD 90（Thy-1）单克隆抗体（MoAbs）在半固体培养基中培养7 d。我们发现Sca-1单克隆抗体抑制集落形成（20-30%），并且这种作用在很大程度上被一氧化氮（NO）合酶抑制剂N-硝基-L-精氨酸甲酯（L-NAME）所消除。用抗CD 90抗体获得了类似的结果。研究结果表明，造血祖细胞上Sca-1和Thy-1标记物的未知生理配体可以抑制集落形成，NO是关键介质。原始祖细胞可能是这种Sca-1配体的主要靶点，因为Sca-1（+）细胞群含有多能造血干细胞的主要部分。
Mouse bone marrow cells were exposed to specific monoclonal antibodies, so that lineage positive (Lin(+)) cells could be removed with magnetic beads. The Lin(-) cells were cultured with Sca-1 or CD90 (Thy-1) monoclonal antibodies (MoAbs) in semi-solid medium for 7 d. We found that Sca-1 MoAb suppressed colony formation (20-30%), and the effect was largely abolished by N-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthase. Similar results were obtained with antibodies to CD90. The findings suggest that the unknown physiological ligands to Sca-1 and Thy-1 markers on haematopoietic progenitor cells can inhibit colony formation, with NO as a pivotal mediator. Primitive progenitors may be a primary target of this Sca-1 ligand, as the Sca-1(+) cell population contains the major part of the multipotent haematopoietic stem cells.