A skeleton-tree-based approach to acinar morphometric analysis using microcomputed tomography with comparison of acini in young and old C57BL/6 mice.
A skeleton-tree-based approach to acinar morphometric analysis using microcomputed tomography with comparison of acini in young and old C57BL/6 mice.
复制标题
使用微型计算机断层扫描进行基于骨架树的腺泡形态测量分析，并比较年轻和年老 C57BL/6 小鼠的腺泡。
DOI：
10.1152/japplphysiol.00923.2015
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Hoffman,EricA
中科院分区：
文献类型：
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作者：
KizhakkePuliyakote,AbhilashS;Vasilescu,DragoşM;SenSharma,Kriti;Wang,Ge;Hoffman,EricA
我们试图建立一种使用内部断层扫描技术(XRadia MicroXCT-400)进行腺泡形态计量学分析的方法，使用来自微型X射线计算机断层扫描(Micro-CT)图像的路径中心线作为路线图。通过应用这些技术，我们提出了一种将小鼠肺图谱扩展到腺泡水平的方法，并对C57BL/6毒株的两个年龄组进行了比较。通过血管灌注固定的肺采用高分辨率Micro-CT扫描。单个腺泡被分割，并形成从腺泡入口处到肺泡囊的骨架路径。生成了包括分支长度、直径和分支角度在内的形态测量参数。研究对象为两个年龄组(∼20周龄和∼90周龄)各6只。使用加性高斯噪声(0均值和SD1、2、5和10)来检验分析方法的稳健性。分支长度的噪声变异在±6μm以内，直径在±5μm以内。当噪声水平为10时，误差增加。树枝直径比长度更不容易受到噪音的影响。在所有的噪声水平上都有95%的中心线重叠。使用中心线作为路线图获得的测量结果不受附加噪声的影响。年轻小鼠的腺泡在所有世代的分枝直径和长度都较小，分枝角度没有明显差异。肺泡管中体积的相对分布在两个年龄组中是相似的。该方法具有良好的重复性和对图像噪声的鲁棒性，为腺泡形态定量评价和比较提供了一种新的非破坏性技术。
We seek to establish a method using interior tomographic techniques (Xradia MicroXCT-400) for acinar morphometric analysis using the pathway center lines from micro X-ray computed tomographic (Micro-CT) images as the road map. Through the application of these techniques, we present a method to extend the atlas of murine lungs to acinar levels and present a comparison between two age groups of the C57BL/6 strain. Lungs fixed via vascular perfusion were scanned using high-resolution Micro-CT protocols. Individual acini were segmented, and skeletonized paths to alveolar sacs from the entrance to the acinus were formed. Morphometric parameters, including branch lengths, diameters, and branching angles, were generated. Six mice each, at two age groups (∼20 and ∼90 wk of age), were studied. Additive Gaussian noise (0 mean and SD 1, 2, 5, and 10) was used to test the robustness of the analytical method. Noise-based variations were within ±6 μm for branch lengths and ±5 μm for diameters. At a noise level of 10, errors increased. Branch diameters were less susceptible to noise than lengths. There was >95% center line overlap across all noise levels. The measurements obtained using the center lines as a road map were not affected by added noise. Acini from younger mice had smaller branch diameters and lengths at all generations without significant differences in branching angles. The relative distribution of volume in the alveolar ducts was similar across both age groups. The method has been demonstrated to be repeatable and robust to image noise and provides a new, nondestructive technique to assess and compare acinar morphometry quantitatively.