Down-regulation of ribosomal protein S15A mRNA with a short hairpin RNA inhibits human hepatic cancer cell growth in vitro
Down-regulation of ribosomal protein S15A mRNA with a short hairpin RNA inhibits human hepatic cancer cell growth in vitro
复制标题
短发夹RNA下调核糖体蛋白S15A mRNA抑制人肝癌细胞体外生长
DOI：
10.1016/j.gene.2013.11.075
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发表时间：
2014-02-15
期刊：
影响因子：
3.5
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Xu, Maiyu;Wang, Yi;Chen, Gang
核糖体蛋白s15 a（RPS 15 A）是一种高度保守的蛋白质，其在翻译早期促进mRNA/核糖体相互作用。最近的证据表明，RPS 15 A可以刺激酵母，植物和人肺癌的生长。在这里，我们报告RPS 15 A敲低可以抑制肝癌细胞在体外的生长。当用含有shRPS 15 A的慢病毒转导时，我们在HepG 2和Bel 7404细胞中观察到细胞增殖抑制和集落形成受损。细胞周期分析显示，转染Lv-shRPS 15 A的HepG 2细胞被阻滞在G 0/G1期。总之，我们的研究结果首次提供了RPS 15 A在肝癌细胞生长中的生物学效应。RPS 15 A可能在肝癌的发生发展中起重要作用，并可能成为肝癌治疗的潜在靶点。(C)2013爱思唯尔有限公司版权所有。
Ribosomal protein s15a (RPS15A) is a highly conserved protein that promotes mRNA/ribosome interactions early in translation. Recent evidence showed that RPS15A could stimulate growth in yeast, plant and human lung carcinoma. Here we report that RPS15A knockdown could inhibit hepatic cancer cell growth in vitro. When transduced with shRPS15A-containing lentivirus, we observed inhibited cell proliferation and impaired colony formation in both HepG2 and Bel7404 cells. Furthermore, cell cycle analysis showed that HepG2 cells were arrested at the G0/G1 phase when transduced with Lv-shRPS15A. In conclusion, our findings provide for the first time the biological effects of RPS15A in hepatic cancer cell growth. RPS15A may play a prominent role in heptocarcinogenesis and serve as a potential therapeutic target in hepatocellular carcinoma. (C) 2013 Elsevier B.V. All rights reserved.