A high sucrose and high fat diet induced the development of insulin resistance in the skeletal muscle of Bama miniature pigs through the Akt/GLUT4 pathway.
A high sucrose and high fat diet induced the development of insulin resistance in the skeletal muscle of Bama miniature pigs through the Akt/GLUT4 pathway.
复制标题
高蔗糖高脂饮食通过Akt/GLUT4途径诱导巴马小型猪骨骼肌胰岛素抵抗的发生
DOI：
10.1538/expanim.17-0010
复制
发表时间：
2017-10-30
影响因子：
2.4
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Liu Y;Yuan J;Xiang L;Zhao Y;Niu M;Dai X;Chen H
高糖高脂（HSHF）饮食可诱导胰岛素抵抗（IR）和2型糖尿病（T2 DM）易感性增加，但其潜在机制尚不清楚。本研究旨在探讨HSHF日粮降低巴马小型猪胰岛素敏感性的分子机制。12头巴马小型猪随机分为对照组（CD组）和HSHF组（n = 6），试验期6个月。测定生化指标。采用Western blot、RT-qPCR和免疫组织化学方法分别检测骨骼肌组织蛋白质、mRNA和葡萄糖转运蛋白4（GLUT 4）表达的变化。与CD组相比，HSHF组的稳态模型评估-胰岛素抵抗（HOMA-IR）指数增加了2.9倍，胰岛素敏感性降低了24.8%。与CD组相比，HSHF组骨骼肌中p-Akt S473降低约59%，GLUT 4降低约43.8%。然而，两组之间p-mTOR S2448的表达无显著差异（P=0.309）。本研究表明，6个月HSHF饮食引起的IR，降低胰岛素敏感性，并减少p-Akt S473和GLUT 4在巴马小型猪骨骼肌中的表达。
A high sucrose and high fat (HSHF) diet induces insulin resistance (IR) and increased susceptibility to type 2 diabetes mellitus (T2DM), but the underlying mechanisms are poorly characterized. This study aimed to investigate the molecular mechanisms by which the HSHF diet impairs insulin sensitivity in Bama miniature pigs (sus scrofa domesticus). Twelve Bama miniature pigs were randomly assigned to the control diet (CD) group (n=6) or the HSHF group (n=6) for 6 months. Biochemical parameters were measured. Western blot, RT-qPCR and immunohistochemistry were used to profile the changes of protein expression, mRNA expression and glucose transporter 4 (GLUT4) expression in skeletal muscle tissues, respectively. In comparison to the CD group, the homeostasis model assessment-insulin resistance (HOMA-IR) index of the HSHF group demonstrated a 2.9-fold increase, and the insulin sensitivity showed a 24.8% decrease. Compared with the CD group, p-Akt S473 decreased by approximately 59% and GLUT4 decreased by 43.8% in the skeletal muscle of the HSHF group. However, the expression of p-mTOR S2448 between the 2 groups was not significantly different (P=0.309). This study demonstrates that a 6-month HSHF diet caused IR, decreased insulin sensitivity, and reduced the expression of p-Akt S473 and GLUT4 in the skeletal muscle of Bama miniature pigs.