Crystallization of Escherichia coli CdtB, the biologically active subunit of cytolethal distending toxin.
Crystallization of Escherichia coli CdtB, the biologically active subunit of cytolethal distending toxin.
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大肠杆菌 CdtB 的结晶，细胞致死膨胀毒素的生物活性亚基。
DOI：
10.1107/s1744309106002454
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Yoder,MarilynD
中科院分区：
文献类型：
--
作者：
Hontz,JillS;Villar-Lecumberri,MariaT;Dreyfus,LawrenceA;Yoder,MarilynD
细胞致死性膨胀毒素(CDT)是一种由多种细菌病原体产生的分泌性蛋白毒素。具有生物活性的CDT亚基CDtB是哺乳动物I型DNA酶的活性同源物。CDtB的内化和随后的移位到靶细胞的核中导致DNA链断裂，导致细胞周期停滞和细胞凋亡。以聚乙二醇8000为沉淀剂，采用微批法生长了CdTb晶体。每不对称单位含1个分子，分子质量为30.5 kDa，属于P212121空间群，衍射峰为1.72 ？
Cytolethal distending toxin (CDT) is a secreted protein toxin produced by several bacterial pathogens. The biologically active CDT subunit CdtB is an active homolog of mammalian type I DNase. Internalization of CdtB and subsequent translocation into the nucleus of target cells results in DNA-strand breaks, leading to cell-cycle arrest and apoptosis. CdtB crystals were grown using microbatch methods with polyethylene glycol 8000 as the precipitant. The CdtB crystals contain one molecule of MW 30.5 kDa per asymmetric unit, belong to space group P212121 and diffract to 1.72 Å.