Comparison of salt tolerance of ectomycorrhizal mushroom Rhizopogon roseolus in soil and on agar
Comparison of salt tolerance of ectomycorrhizal mushroom Rhizopogon roseolus in soil and on agar
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DOI：
10.24465/msb.25.1_17
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Q. Gao;S. Nakano;T. Aimi;N. Shimomura
中科院分区：
文献类型：
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作者：
Q. Gao;S. Nakano;T. Aimi;N. Shimomura
蔷薇根菌（Rhizopogon roseolus）是松科重要的外生菌根共生体，是一种下生担子菌，日语称“shoro”。近年来的研究表明，外生菌根真菌在保护寄主根系免受重金属和过量盐等环境胁迫方面发挥着重要作用。然而，目前还没有研究表明玫瑰红霉耐盐菌株在土壤基质中的特性。本研究利用野外沙质土壤对玫瑰红霉菌菌株在土壤中的生长情况进行了观察，并分析了菌丝生长与土壤基质和琼脂培养基之间的相关性。所有菌株在没有寄主植物的沙质土壤中生长良好，土壤表面菌丝聚集形成菌丝菌落。在土壤和琼脂上，红毛霉菌丝生长呈极显著的正相关。在盐水培养中，在100%海水盐度的土壤中菌丝生长与在100%海水琼脂培养基上菌丝生长呈正相关。这些结果表明，土壤中玫瑰红霉菌菌丝体对盐的敏感性高于琼脂，砂质土壤可用于表征玫瑰红霉菌菌株在土壤基质中的耐盐性。
Rhizopogon roseolus , a hypogeous basidiomycete, also known as “shoro” in Japan, is an important ectomycorrhizal symbiont of the Pinaceae. Recent studies suggest that ectomycorrhizal fungi play an important role in the protection of host roots from environmental stressors such as heavy metals and excessive salt. However, there are no studies characterizing salt tolerant strains of R. roseolus in a soil substrate. Here, we used sandy soil from the wild to observe the growth of R. roseolus strains in soil, and analyzed the correlation of mycelial growth between soil substrate and agar medium. All the strains grew well in sandy soil without a host plant, and on the surface of the soil, several hyphae aggregated to form a mycelial colony. A significant positive correlation was observed between the mycelial growth of R. roseolus in soil and on agar. In saline culture, mycelial growth in soil with 100% of the salinity of seawater showed a positive correlation with mycelial growth on 100% seawater agar medium. These results suggest that mycelia of R. roseolus seem more sensitive to salt in soil than on agar, and sandy soil will be useful to characterize the salt tolerance of R. roseolus strains in soil substrates.