Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei.
Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei.
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DOI：
10.1186/1471-2334-8-170
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发表时间：
2008-12-22
影响因子：
3.7
通讯作者：
Lu JJ
中科院分区：
文献类型：
--
作者：
Dou HY;Tseng FC;Lin CW;Chang JR;Sun JR;Tsai WS;Lee SY;Su IJ;Lu JJ
人口稠密城市的结核病控制因人与人之间的密切接触和病原体可能从多种来源传播而变得复杂。我们对台北市肺结核病人的356株结核分枝杆菌(MTB)进行了分子流行病学分析。培养后进行经典的抗菌谱研究和基因特征分析，采用分枝杆菌散布重复单位可变数目串联重复序列(Miru-VNTR)分型和SPOGO分型。对356株沙门氏菌进行了标准基因分型，并与国际标准基因分型数据库(SpolDB4)进行了比较。采用15个MIRU-VNTR基因座分型方法，结合NTF基因座和RD缺失分析，对所有分离株进行分类。在356株分离株中，290株(81.4%)显示了已知的球型，66株未在数据库中鉴定。北京家系占52.5%，其次是Haarlem家系(13.5%)和EAI+EAI样家系(11%)。当使用MIRU-VNTR时，共鉴定出140种模式，其中包括36个由252个分离物组成的聚类群和104个独特模式，其中最大的聚类群由来自北京的95个分离物组成。SPOGO分型和Miru-VNTR相结合显示，356株分离物中236株(67%)聚集在43种基因型别上。北京家系的菌株更可能为现代菌株，多重耐药率高于其他家系的总和(P=0.08)。北京株感染的患者比其他株感染的患者年轻(平均58.7vs.64.2，p=0.02)。此外，在25岁以下的感染者中，85.3%的人感染了北京现代菌株，这表明台北最近可能存在这种结核菌株家族在年轻人群中传播的情况。我们在台北地区的结核分枝杆菌基因分型资料显示，结核分枝杆菌感染尚未得到最佳控制。鉴于北京株在年轻人群中的高流行率以及与耐药性有关，应加强控制工作。
The control of tuberculosis in densely populated cities is complicated by close human-to-human contacts and potential transmission of pathogens from multiple sources. We conducted a molecular epidemiologic analysis of 356 Mycobacterium tuberculosis (MTB) isolates from patients presenting pulmonary tuberculosis in metropolitan Taipei. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and spoligotyping, were applied after culture. A total of 356 isolates were genotyped by standard spoligotyping and the strains were compared with in the international spoligotyping database (SpolDB4). All isolates were also categorized using the 15 loci MIRU-VNTR typing method and combin with NTF locus and RD deletion analyses. Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not identified in the database. Major spoligotypes found were Beijing lineages (52.5%), followed by Haarlem lineages (13.5%) and EAI plus EAI-like lineages (11%). When MIRU-VNTR was employed, 140 patterns were identified, including 36 clusters by 252 isolates and 104 unique patterns, and the largest cluster comprised 95 isolates from the Beijing family. The combination of spoligotyping and MIRU-VNTR revealed that 236 (67%) of the 356 isolates were clustered in 43 genotypes. Strains of the Beijing family was more likely to be of modern strain and a higher percentage of multiple drug resistance than other families combined (P = 0.08). Patients infected with Beijing strains were younger than those with other strains (mean 58.7 vs. 64.2, p = 0.02). Moreover, 85.3% of infected persons younger than 25 years had Beijing modern strain, suggesting a possible recent spread in the young population by this family of TB strain in Taipei. Our data on MTB genotype in Taipei suggest that MTB infection has not been optimally controlled. Control efforts should be reinforced in view of the high prevalence of the Beijing strain in young population and association with drug resistance.
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DOI：
10.1073/pnas.98.4.1901
发表时间：
2001-02-13
影响因子：
11.1
作者：
Mazars, E;Lesjean, S;Supply, P
通讯作者：
Supply, P
影响因子：
5
作者：
Murray, M;Alland, D
通讯作者：
Alland, D
影响因子：
158.5
作者：
ALLAND, D;KALKUT, GE;BLOOM, BR
通讯作者：
BLOOM, BR
结核分枝杆菌复杂的遗传多样性：挖掘第四个国际螺旋型数据库（SpoldB4），用于分类，种群遗传学和流行病学。
影响因子：
4.2
作者：
Brudey K;Driscoll JR;Rigouts L;Prodinger WM;Gori A;Al-Hajoj SA;Allix C;Aristimuño L;Arora J;Baumanis V;Binder L;Cafrune P;Cataldi A;Cheong S;Diel R;Ellermeier C;Evans JT;Fauville-Dufaux M;Ferdinand S;Garcia de Viedma D;Garzelli C;Gazzola L;Gomes HM;Guttierez MC;Hawkey PM;van Helden PD;Kadival GV;Kreiswirth BN;Kremer K;Kubin M;Kulkarni SP;Liens B;Lillebaek T;Ho ML;Martin C;Martin C;Mokrousov I;Narvskaïa O;Ngeow YF;Naumann L;Niemann S;Parwati I;Rahim Z;Rasolofo-Razanamparany V;Rasolonavalona T;Rossetti ML;Rüsch-Gerdes S;Sajduda A;Samper S;Shemyakin IG;Singh UB;Somoskovi A;Skuce RA;van Soolingen D;Streicher EM;Suffys PN;Tortoli E;Tracevska T;Vincent V;Victor TC;Warren RM;Yap SF;Zaman K;Portaels F;Rastogi N;Sola C
通讯作者：
Sola C
DOI：
10.1073/pnas.052548299
发表时间：
2002-03-19
影响因子：
11.1
作者：
Brosch, R;Gordon, SV;Cole, ST
通讯作者：
Cole, ST