Paludibacter jiangxiensis sp nov., a strictly anaerobic, propionate-producing bacterium isolated from rice paddy field
Paludibacter jiangxiensis sp nov., a strictly anaerobic, propionate-producing bacterium isolated from rice paddy field
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DOI：
10.1007/s00203-013-0951-1
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发表时间：
2014-03-01
影响因子：
2.8
通讯作者：
Guo, Rong-Bo
中科院分区：
文献类型：
--
作者：
Qiu, Yan-Ling;Kuang, Xiao-Zhu;Guo, Rong-Bo
从农村稻田中分离到一株嗜中温、专性厌氧的丙酸发酵菌，命名为NM 7（T）。菌株NM 7（T）的细胞为革兰氏阴性、不运动、不形成孢子、短杆状，并且对过氧化氢酶呈阴性。该菌株在37 A ° C（生长范围15-40 A ° C）和pH 7.0（pH 5.0-7.5）下生长最佳。该菌株能发酵多种糖类，包括阿拉伯糖、木糖、果糖、半乳糖、葡萄糖、甘露糖、纤维二糖、乳糖、麦芽糖、蔗糖、果胶和淀粉。葡萄糖发酵的主要终产物是乙酸和丙酸。酵母提取物是不需要的，但刺激生长。硝酸盐、硫酸盐、硫代硫酸盐、元素硫、亚硫酸盐和Fe（III）次氮基三乙酸盐未用作末端电子受体。基因组DNA的G+C含量为42.8mol%。主要的细胞脂肪酸为C-15：0、antiso-C-15：0、C-16：0和C-17：0。菌株NM 7（T）的极性脂质以磷脂酰乙醇胺含量最高。16 S rRNA基因序列分析表明，该菌属于拟杆菌门（Bacteroidetes）的紫单胞菌科（Porphyromonadaceae）。最接近的识别种是产丙酸沼泽杆菌（16 S rRNA基因序列相似性为91.4%）。一新种，江西沼杆菌Paludibacter jiangxiensis sp. nov.，建议适应菌株NM 7（T）（=JCM 17480（T）= CGMCC 1.5150（T）= KCTC 5844（T））。
A mesophilic, obligately anaerobic, propionate-producing fermentative bacterium, designated strain NM7(T), was isolated from rural rice paddy field. Cells of strain NM7(T) are Gram-negative, non-motile, non-spore-forming, short rods, and negative for catalase. The strain grew optimally at 37 A degrees C (the range for growth 15-40 A degrees C) and pH 7.0 (pH 5.0-7.5). The strain could grow fermentatively on various sugars, including arabinose, xylose, fructose, galactose, glucose, mannose, cellobiose, lactose, maltose, sucrose, pectin and starch. The main end products of glucose fermentation were acetate and propionate. Yeast extract was not required but stimulated the growth. Nitrate, sulfate, thiosulfate, elemental sulfur, sulfite, and Fe(III) nitrilotriacetate were not used as terminal electron acceptors. The G+C content of genomic DNA was 42.8 mol%. The major cellular fatty acids were C-15:0, anteiso-C-15:0, C-16:0, and C-17:0. The most abundant polar lipid of strain NM7(T) was phosphatidylethanolamine. 16S rRNA gene sequence analysis revealed that it belongs to the family Porphyromonadaceae of the phylum Bacteroidetes. The closest recognized species was Paludibacter propionicigenes (91.4 % similarity in 16S rRNA gene sequence). A novel species, Paludibacter jiangxiensis sp. nov., is proposed to accommodate strain NM7(T) (=JCM 17480(T) = CGMCC 1.5150(T) = KCTC 5844(T)).