Profiling of cell stress proteins reveals decreased expression of enzymatic antioxidants in tracheal epithelial tissue of pigs raised indoors.
Profiling of cell stress proteins reveals decreased expression of enzymatic antioxidants in tracheal epithelial tissue of pigs raised indoors.
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DOI：
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发表时间：
2019
影响因子：
2.2
通讯作者：
Jenora T Waterman;C. J. McClendon;Rohit S Ranabhat;Ke'Yona T Barton
中科院分区：
文献类型：
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作者：
Jenora T Waterman;C. J. McClendon;Rohit S Ranabhat;Ke'Yona T Barton
暴露于室内猪生产设施（SPF）环境会导致设施工人的气道炎症和疾病，包括哮喘、慢性支气管炎和慢性阻塞性肺病（COPD）。然而，人们对SPF暴露对猪呼吸道健康的影响知之甚少。呼吸道症状与反复暴露于SPF有关，特别是吸入有机粉尘和其他空气污染物。要了解气道炎症和慢性炎症性肺病，需要彻底了解SPF暴露调节的分子途径。本研究测定了在SPF或牧场饲养的猪的气管上皮组织中与氧化应激和抗氧化防御相关的蛋白质的表达。利用蛋白质组分析仪、细胞应激阵列、蛋白质印迹和酶活性测定来测量猪气管上皮组织提取物中的蛋白质表达和活性水平。经测定，与牧场饲养的猪相比，SPF饲养的猪在其气管上皮组织内表达显著较少的酶抗氧化剂，包括超氧化物歧化酶（SOD）。与此同时，SPF养猪的气管上皮组织中的SOD和过氧化氢酶的抗氧化活性水平较低的牧场饲养的猪。本文总结的观察结果提供的证据表明，暴露于猪生产环境影响猪气管上皮组织内的内源性酶抗氧化防御。这项研究提供了深入了解持续暴露于SPF污染物对气道上皮内源性抗氧化防御的影响，并可能有助于了解人类气道对猪舍暴露的反应。
Exposure to indoor swine production facilities (SPF) environments causes airway inflammation and diseases including asthma, chronic bronchitis and chronic obstructive pulmonary disease (COPD) in facility workers. However, less is known about the impact of SPF exposures on the respiratory health of pigs. Respiratory symptoms are associated with repeat exposure to SPF, specifically inhalation of organic dust and other air pollutants therein. A thorough understanding of the molecular pathways regulated by SPF exposure is needed to understand airway inflammation and chronic inflammatory lung disease. The present study measured the expression of proteins associated with oxidative stress and antioxidant defenses in the tracheal epithelial tissues of pigs reared in SPF or on pasture. Proteome profiler cell stress arrays, western blotting and enzyme activity assays were utilized to measure protein expression and activity levels in tracheal epithelial tissue extracts of pigs. It was determined that pigs raised in SPF express significantly less enzymatic antioxidants, including superoxide dismutase (SOD), within their tracheal epithelial tissues compared to pasture raised pigs. Concomitantly, tracheal epithelial tissues of SPF raised pigs had lower SOD and catalase antioxidant activity levels compared to pasture raised pigs. The observations summarized herein provide evidence that exposure to swine production environments influence endogenous enzymatic antioxidant defenses within the tracheal epithelial tissues of pigs. This study offers insight for understanding the effect of continuous exposure to SPF pollutants on endogenous antioxidant defenses in the airway epithelial and may be helpful in understanding human airway responses to swine barn exposures.