Competitive interactions of ligands and macromolecular crowders with maltose binding protein.
Competitive interactions of ligands and macromolecular crowders with maltose binding protein.
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DOI：
10.1371/journal.pone.0074969
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhou HX
中科院分区：
文献类型：
--
作者：
Miklos AC;Sumpter M;Zhou HX
细胞信号涉及在蛋白质、多糖和其他大分子浓度较高的复杂环境中发生的一系列识别事件。大分子聚集体通过硬核排斥力对蛋白质结合亲和力的影响已经得到了很好的研究，最近已经暗示了蛋白质-Crowder软吸引的可能贡献。在这里，我们提出了麦芽糖结合蛋白(MBP)与多糖Crowder Ficoll弱结合的直接证据，并且这种结合有效地与天然配体麦芽糖的结合竞争。麦芽糖和无花果浓度范围内的滴定数据符合三态竞争结合模型。加入Ficoll使MBP的1Hü15N TROSY谱变宽，表明蛋白质与Crowder的结合很弱，添加麦芽糖后恢复了尖锐的核磁共振峰，表明Crowder及其配体与MBP的相互作用是竞争性的。我们推测，在大肠杆菌周质中，多糖和麦芽糖与MBP的竞争性相互作用可以使MBP在附着在外膜上的肽聚糖和内膜的ATP结合盒转运蛋白之间穿梭。
Cellular signaling involves a cascade of recognition events occurring in a complex environment with high concentrations of proteins, polysaccharides, and other macromolecules. The influence of macromolecular crowders on protein binding affinity through hard-core repulsion is well studied, and possible contributions of protein-crowder soft attraction have been implicated recently. Here we present direct evidence for weak association of maltose binding protein (MBP) with a polysaccharide crowder Ficoll, and that this association effectively competes with the binding of the natural ligand, maltose. Titration data over wide ranges of maltose and Ficoll concentrations fit well with a three-state competitive binding model. Broadening of MBP 1H15N TROSY spectra by the addition of Ficoll indicates weak protein-crowder association, and subsequent recovery of sharp NMR peaks upon addition of maltose indicates that the interactions of the crowder and the ligand with MBP are competitive. We hypothesize that, in the Escherichia coli periplasm, the competitive interactions of polysaccharides and maltose with MBP could allow MBP to shuttle between the peptidoglycan attached to the outer membrane and the ATP-binding cassette transporter in the inner membrane.
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影响因子：
13.8
作者：
Ladbury, John E.;Arold, Stefan T.
通讯作者：
Arold, Stefan T.
有吸引力的蛋白质-聚合物相互作用显着改变大分子拥挤对蛋白质缔合平衡的影响
影响因子：
3.4
作者：
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影响因子：
3.2
作者：
Arends, S. J. Ryan;Williams, Kyle;Weiss, David S.
通讯作者：
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影响因子：
3.2
作者：
BRASS, JM;HIGGINS, CF;GARLAND, PB
通讯作者：
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影响因子：
2.9
作者：
Benton, Laura A.;Smith, Austin E.;Pielak, Gary J.
通讯作者：
Pielak, Gary J.