Prostaglandin endoperoxide synthase (cyclooxygenase): ultrastructural localization to nonmembrane-bound cytoplasmic lipid bodies in human eosinophils and 3T3 fibroblasts.
Prostaglandin endoperoxide synthase (cyclooxygenase): ultrastructural localization to nonmembrane-bound cytoplasmic lipid bodies in human eosinophils and 3T3 fibroblasts.
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前列腺素内过氧化物合酶（环氧合酶）：人嗜酸性粒细胞和 3T3 成纤维细胞中非膜结合细胞质脂质体的超微结构定位。
DOI：
10.1159/000236764
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发表时间：
1994
影响因子：
2.8
通讯作者：
Weller,PF
中科院分区：
文献类型：
--
作者：
Dvoràk,AM;Morgan,ES;Tzizik,DM;Weller,PF
脂质体是非膜结合的细胞质包涵体，在参与炎症反应的细胞中表现突出。用抗前列腺素内过氧化物合成酶单克隆抗体包埋后免疫金定位，在电镜下将PGH合成酶定位于两种前列腺素形成细胞（人嗜酸性粒细胞和鼠3T3成纤维细胞）的脂质体上。研究了高嗜酸性粒细胞综合征供者新鲜分离的嗜酸性粒细胞和与3T3成纤维细胞和粒细胞-巨噬细胞集落刺激因子共培养的外周血嗜酸性粒细胞。在这两种细胞类型中，脂质体是抗pgh合成酶标记的主要结构。对照组没有标签。在人类嗜酸性粒细胞和小鼠成纤维细胞中，脂质体代表了PGH合成酶（前列腺素形成的限速初始酶）的定位位点，并可能作为环氧化酶途径衍生的二十烷类化合物形成的特定位点。
Lipid bodies are nonmembrane-bound cytoplasmic inclusions which are prominent in cells engaged in inflammatory responses. Using postembedding immunogold localization with a primary anti-PGH (prostaglandin endoperoxide) synthase monoclonal antibody, PGH synthase was localized by electron microscopy to lipid bodies of two prostaglandin-forming cells: human eosinophils and murine 3T3 fibroblasts. Freshly isolated eosinophils from a hypereosinophilic syndrome donor and peripheral blood eosinophils maintained in coculture with 3T3 fibroblasts and granulocyte-macrophage colony-stimulating factor were studied. In both cell types, lipid bodies were the predominant structures labeled with anti-PGH synthase. Labeling was absent in controls. In both human eosinophils and murine fibroblasts, lipid bodies represent sites of localization of PGH synthase – the rate-limiting initial enzyme in the formation of prostaglandins – and may serve as specific sites of formation of cyclooxygenase-pathway-derived eicosanoids.