A candidate target for G protein action in brain
A candidate target for G protein action in brain
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DOI：
10.1074/jbc.274.38.26931
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发表时间：
1999-09-17
影响因子：
4.8
通讯作者：
Kozasa, T
中科院分区：
文献类型：
--
作者：
Chen, LT;Gilman, AG;Kozasa, T
以磷酸化的S-G(Z)α为探针，通过筛选表达文库，确定了G蛋白作用的效应候选基因GRIN1。GRIN1是一种新的蛋白质，与已知的蛋白质结构域没有实质性的同源性。它主要在大脑中表达，并通过其羧基末端区域与激活的G(Z)α、G(O)α和G(I)α特异结合。蛋白KIAA0514(GRIN2)在其羧基末端与GRIN1同源，并与活化的G(O)α结合。GRIN1和G(O)α都是膜结合蛋白，富含在神经突起的生长锥体中。GRIN1或GRIN2与激活的G(O)α共表达可在Neuro2a细胞中形成一个精细突起网络，提示这些途径可能在G(O)α下游发挥作用，以控制神经突起的生长。
An effector candidate for G protein action, GRIN1, was identified by screening a cDNA expression library with phosphorylatled GTP gamma S-G(z)alpha as a probe. GRIN1 is a novel protein without substantial homology to known protein domains. It is expressed largely in brain and binds specifically to activated G(z)alpha, G(o)alpha, and G(i)alpha through its carboxyl-terminal region. The protein KIAA0514 (GRIN2) is homologous to GRIN1 at its carboxyl terminus and also binds to activated G(o)alpha. Both GRIN1 and G(o)alpha are membrane-bound proteins that are enriched in the growth cones of neurites. Coexpression of GRIN1, or GRIN2 with activated G(o)alpha causes formation of a network of fine processes in Neuro2a cells, suggesting that these pathways may function downstream of G(o)alpha to control growth of neurites.