Heteromeric connexons in lens gap junction channels
Heteromeric connexons in lens gap junction channels
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DOI：
10.1073/pnas.93.3.1287
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发表时间：
1996-02-06
影响因子：
11.1
通讯作者：
Goodenough, DA
中科院分区：
文献类型：
--
作者：
Jiang, JX;Goodenough, DA
间隙连接通道是由一对称为连接子的寡聚膜半通道形成的，连接子由连接蛋白家族的蛋白质组成。对转染细胞系和配对的非洲爪蟾卵母细胞的实验表明，由两个连接子组成的异型细胞间通道可以选择性地发生，每个连接子由不同的连接蛋白组成。Stauffer的研究[Stauffer， K. A. (1995) J. Biol, Chem. 270,6768 -6772]表明重组Cx26和Cx32共感染昆虫细胞可形成异聚连接子。通过溶解和免疫沉淀，连接子可以包含两种不同的连接子，在体内形成异质组装体。
Gap junction channels are formed by paired oligomeric membrane hemichannels called connexons, which are composed of proteins of the connexin family. Experiments with transfected cell lines and paired Xenopus oocytes have demonstrated that heterotypic intercellular channels which are formed by two connexons, each composed of a different connexin, can selectively occur. Studies by Stauffer [Stauffer, K. A. (1995) J. Biol, Chem. 270, 6768-6772] have shown that recombinant Cx26 and Cx32 coinfected into insect cells may form heteromeric connexons. By solubilizing and subfraction-immunoprecipitation that connexons can contain two different connexins forming heteromeric assemblies in vivo.