ELECTROSPRAY AND TAYLOR-CONE THEORY, DOLES BEAM OF MACROMOLECULES AT LAST
ELECTROSPRAY AND TAYLOR-CONE THEORY, DOLES BEAM OF MACROMOLECULES AT LAST
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DOI：
10.1016/0168-1176(94)04024-9
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发表时间：
1994-09-22
影响因子：
1.8
通讯作者：
MANN, M
中科院分区：
文献类型：
--
作者：
WILM, MS;MANN, M
对电喷雾中的静电分散进行了理论描述。该理论预测了泰勒锥尖端喷射液体的区域的大小。为了最大限度地减少这个发射区的修改后的电喷雾离子源，微电喷雾，已建成。利用该离子源，液滴的尺寸在200 nm范围内或更小。喷雾非常稳定，因此水溶液可以在没有任何辅助的情况下以负或正模式分散。微电喷雾离子源在使用所施加的样品量方面非常有效。它的流速小于25 nl/min，使0.5 μ l样品的MS-MS研究成为可能。已测得肽从溶液状态到检测器的总透射系数为8 × 10 ~（-4）。
A theoretical description of the electrostatic dispersion in electrospray is presented. The theory predicts the size of the zone at the tip of the Taylor Cone from which the liquid is ejected. To minimize this emission zone a modified electrospray ion source, the micro electrospray, has been constructed. With this ion source the size of the droplets is in the 200 nm range or below. The spray is very stable so that aqueous solutions can be dispersed in negative or positive mode without any assistance. The micro electrospray ion source is very efficient in the use of the applied amount of sample. Its flow rate of less than 25 nl/min makes MS-MS investigations on 0.5 mu l of sample possible. An overall transmission coefficient of 8 x 10(-4) for a peptide from the solution state to the detector has been measured.