A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality.
A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality.
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DOI：
10.1039/c4cc05554c
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发表时间：
2014-10
影响因子：
4.9
通讯作者：
Zhenpeng Zhou;Tian Li;Hongduan Huang;Yang Chen;Fen Liu;C. Huang;Na Li
中科院分区：
文献类型：
--
作者：
Zhenpeng Zhou;Tian Li;Hongduan Huang;Yang Chen;Fen Liu;C. Huang;Na Li
Silver-enhanced fluorescence was coupled with a bio-barcode assay to facilitate a dual amplification assay to demonstrate a non-enzymatic approach for simple and sensitive detection of DNA. In the assay design, magnetic nanoparticles seeded with silver nanoparticles were modified with the capture DNA, and silver nanoparticles were modified with the binding of ssDNA and the fluorescently labeled barcode dsDNA. Upon introduction of the target DNA, a sandwich structure was formed because of the hybridization reaction. By simple magnetic separation, silver-enhanced fluorescence of barcode DNAs could be readily measured without the need of a further step to liberate barcode DNAs from silver nanoparticles, endowing the method with simplicity and high sensitivity with a detection limit of 1 pM.
