Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis
Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis
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DOI：
10.1093/humrep/16.9.1802
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发表时间：
2001-09-01
影响因子：
6.1
通讯作者：
Braun, DP
中科院分区：
文献类型：
--
作者：
Dmowski, WP;Ding, J;Braun, DP
背景：子宫内膜异位症的病因尚不清楚。子宫内膜细胞的异位播散是子宫内膜异位病变的起源，但发生在有或无子宫内膜异位症的妇女中。已经表明增加的异位细胞存活促进它们的植入。本研究的目的是评估子宫内膜异位症妇女的子宫内膜凋亡，根据：（i）周期性变化，（ii）腺体和间质的贡献，（iii）疾病的阶段。方法：受试者为因疑似子宫内膜异位症接受诊断性腹腔镜检查和子宫内膜活检的妇女。使用TdT介导的dUTP-生物素缺口末端标记（TUNEL）测定法评价自发性细胞凋亡。对5 μ m子宫内膜组织切片中10-50 mm（2）区域内每10 mm（2）的凋亡细胞（凋亡指数）进行计数，并记录这些细胞的位置。研究结果：子宫内膜异位症组腺上皮细胞凋亡指数低于对照组（26.0 +/- 5.5 vs 51.2 +/- 9.7，P = 0.03），但间质细胞凋亡指数不低于对照组（36.3 +/- 6.4 vs 48.4 +/- 11.3，NS）。在对照组中，细胞凋亡是最高的分泌/月经后期和早期增殖阶段和周期变异是明显的。在子宫内膜异位症中，这种周期性变异消失了。随着疾病分期的增加，细胞凋亡有减少的趋势，但差异缺乏统计学意义。结论：子宫内膜异位症女性的子宫内膜腺体自发性细胞凋亡减少，特别是在周期的分泌/月经后期和早期增殖期。这可能表明月经期间脱落的子宫内膜细胞的活力增加，促进其异位存活和植入。
BACKGROUND: The aetiology of endometriosis is unknown. Ectopic dissemination of the endometrial cells gives origin to endometriotic lesions, but occurs in women with and without endometriosis. It has been suggested that increased ectopic cell survival facilitates their implantation. The objectives of this study were to evaluate endometrial apoptosis in women with endometriosis according to: (i) cyclic changes, (ii) glandular and stromal contribution, and (iii) stage of the disease. METHODS: The subjects were women undergoing diagnostic laparoscopy and endometrial biopsies for suspected endometriosis. Spontaneous apoptosis was evaluated using TdT-mediated dUTP-biotin nick end-labelling (TUNEL) assay. Apoptotic cells per 10 mm(2) (apoptotic index) in an area of 10-50 mm(2) in 5 mum endometrial tissue sections were counted and location of these cells was recorded. RESULTS: The apoptotic index in glandular epithelium was lower in endometriosis than controls (26.0 +/- 5.5 versus 51.2 +/- 9.7, P = 0.03) but not in the stroma (36.3 +/- 6.4 versus 48.4 +/- 11.3, NS). In controls, apoptosis was highest during the late secretory/menstrual and early proliferative phases and cyclic variability was apparent. In endometriosis, this cyclic variability was lost. There was a trend toward decreased apoptosis with increasing stage of the disease, but the differences lacked statistical significance. CONCLUSIONS: Spontaneous apoptosis is decreased in the endometrial glands in women with endometriosis, especially during late secretory/menstrual and early proliferative phases of the cycle. This may indicate increased viability of endometrial cells shed during menses, facilitating their ectopic survival and implantation.