Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A
Amperometric biosensor based on tyrosinase immobilized onto multiwalled carbon nanotubes-cobalt phthalocyanine-silk fibroin film and its application to determine bisphenol A
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DOI：
10.1016/j.aca.2009.11.051
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发表时间：
2010-02-05
影响因子：
6.2
通讯作者：
Zhu, Lusheng
中科院分区：
文献类型：
--
作者：
Yin, Huanshun;Zhou, Yunlei;Zhu, Lusheng
将酪氨酸酶固定在多壁碳纳米管（MWNTs）-酞菁钴（CoPc）-丝素蛋白（SF）复合修饰玻碳电极（GCE）上，制备了电流型双酚A（BPA）生物传感器。在MWNTs-CoPc-SF复合膜中，SF为酪氨酸酶提供了生物相容的微环境，使其保持生物活性; MWNTs具有优良的固有导电性，提高了电子传递速率; CoPc对BPA的电氧化表现出良好的电催化活性。BPA在该电极上的循环伏安曲线在0.625 V处有一个明显的阳极峰，与裸电极相比，BPA的氧化信号明显增强，因此，利用该氧化信号测定BPA。对影响因素进行了优化，并计算了电化学参数。并对可能的氧化机理进行了讨论。在最佳条件下。氧化电流与BPA浓度在5.0 × 10 ~（-8）~ 3.0 × 10 ~（-6）M范围内呈良好的线性关系，相关系数为0.9979，检出限为3.0 × 10 ~（-8）M（S/N = 3）。方法用于塑料制品中双酚A的测定，回收率在95.36%~ 104.39%之间。(C)2009爱思唯尔有限公司版权所有。
An amperometric bisphenol A (BPA) biosensor was fabricated by immobilizing tyrosinase on multiwalled carbon nanotubes (MWNTs)-cobalt phthalocyanine (CoPc)-silk fibroin (SF) composite modified glassy carbon electrode (GCE). In MWNTs-CoPc-SF composite film, SF provided a biocompatible microenvironment for the tyrosinase to retain its bioactivity, MWNTs possessed excellent inherent conductivity to enhance the electron transfer rate and CoPc showed good electrocatalytic activity to electrooxidation of BPA. The cyclic voltammogram of BPA at this biosensor exhibited a well defined anodic peak at 0.625 V. Compared with bare GCE, the oxidation signal of BPA significantly increased; therefore, this oxidation signal was used to determine BPA. The effect factors were optimized and the electrochemical parameters were calculated. The possible oxidation mechanism was also discussed. Under optimum conditions. the oxidation current was proportional to BPA concentration in the range from 5.0 x 10(-8) to 3.0 x 10(-6) M with correlation coefficient of 0.9979 and detection limit of 3.0 x 10(-8) M (S/N = 3). The proposed method was successfully applied to determine BPA in plastic products and the recovery was in the range from 95.36% to 104.39%. (C) 2009 Elsevier B.V. All rights reserved.