Microsatellite genotyping of carnation varieties
Microsatellite genotyping of carnation varieties
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DOI：
10.1007/s00122-002-1166-z
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发表时间：
2003-05-01
影响因子：
5.4
通讯作者：
Vosman, B
中科院分区：
文献类型：
--
作者：
Smulders, MJM;Noordijk, Y;Vosman, B
使用富含微卫星的 DNA 文库开发了一组 11 个康乃馨 (Dianthus caryophyllus) 序列标记的微卫星标记。辅以来自序列数据库条目的三个标记，这些标记被用于使用半自动基于荧光的方法对康乃馨品种进行基因分型。在一组 82 个品种中，每个标记扩增了 4-16 个等位基因。等位基因的有效数量从1.9到6.0不等。对于八个最佳评分标记，杂合度在 0.51 到 0.99 之间。这些标记能够区分具有独特等位基因组合的所有品种，但运动突变体除外，它们很容易与原始品种归为一组。此外，一组三个品种和一组六个品种各自具有相同的“等位基因峰”组合。三个品种的簇涉及原始品种及其突变体。这六个突变体由来自同一品种的四个突变体和两个其他品种组成。
A set of 11 sequence-tagged microsatellite markers for carnation (Dianthus caryophyllus) was developed using a DNA library enriched for microsatellites. Supplemented with three markers derived from sequence database entries, these were used to genotype carnation varieties using a semi-automated fluorescence-based approach. In a set of 82 cultivars, the markers amplified 4-16 alleles each. The effective number of alleles varied from 1.9 to 6.0. For the eight best scorable markers, heterozygosity was between 0.51 and 0.99. The markers were able to distinguish all cultivars with a unique combination of alleles, except for sport mutants, which were readily grouped together with the original cultivar. In addition, one group of three and one group of six cultivars each had the same combination of 'allelic peaks'. The cluster of three varieties concerned original cultivars and their mutants. The cluster of six consisted of four mutants from the same cultivar and two other varieties.