Biodegradation of nicotine by a newly isolated Agrobacterium sp strain S33
Biodegradation of nicotine by a newly isolated Agrobacterium sp strain S33
复制标题
新分离的农杆菌对尼古丁的生物降解。
DOI：
10.1111/j.1365-2672.2009.04259.x
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发表时间：
2009-09-01
影响因子：
4
通讯作者：
Xu, P.
中科院分区：
文献类型：
--
作者：
Wang, S. N.;Liu, Z.;Xu, P.
目的：从烟草植物根际土壤中分离和鉴定具有降解尼古丁能力的细菌，并利用其降解烟草固体废物中的尼古丁。方法与结果：从烟草植物根际土壤中新分离到一株细菌，菌株S33，经鉴定为农杆菌。基于形态学、生理学测试、Biolog MicroLog3 4中心点20系统和16S rRNA基因序列。以尼古丁作为培养基中唯一的碳源和氮源，在30℃和pH 7中心点0的条件下，在1中心点0 g l(-1)尼古丁条件下生长最佳，尼古丁在6 h内完全降解。葡萄糖铵培养基或LB培养基制备的静息细胞在10 h内不能降解尼古丁，而尼古丁培养基制备的静息细胞可在1中心点5 h内完全降解3 g l(-1)尼古丁，最大降解率为1中心点23 g尼古丁h(-1) g(-1)干细胞。采用同时含有烟碱、葡萄糖和铵的培养基培养菌株S33，静息细胞可在7 h内降解浓度为30 mg烟碱g(-1)干重烟草固体废物中98中心点87%的烟碱，最大降解率为0中心点46 g烟碱h(-1) g(-1)干细胞。具有降解尼古丁的能力。农杆菌属S33可以使用尼古丁作为碳和氮的唯一来源。利用烟碱-葡萄糖-铵培养基制备的菌株S33的静息细胞是降解烟碱和烟草固体废物解毒的有效方法。 研究意义和影响：烟草废物中的烟碱对人体健康和环境有毒有害。这项研究表明农杆菌。 S33可能适合处理烟草废物并降低烟叶中的尼古丁含量。
Aims:To isolate and characterize bacteria capable of degrading nicotine from the rhizospheric soil of a tobacco plant and to use them to degrade the nicotine in tobacco solid waste.Methods and Results:A bacterium, strain S33, was newly isolated from the rhizospheric soil of a tobacco plant, and identified as Agrobacterium sp. based on morphology, physiological tests, Biolog MicroLog3 4 center dot 20 system and 16S rRNA gene sequence. Using nicotine as the sole source of carbon and nitrogen in the medium, it grew optimally with 1 center dot 0 g l(-1) of nicotine at 30 degrees C and pH 7 center dot 0, and nicotine was completely degraded within 6 h. The resting cells prepared from the glucose-ammonium medium or LB medium could not degrade nicotine within 10 h, while those prepared from the nicotine medium could completely degrade 3 g l(-1) of nicotine in 1 center dot 5 h at a maximal rate of 1 center dot 23 g nicotine h(-1) g(-1) dry cell. Using the medium containing nicotine, glucose and ammonium simultaneously to cultivate strain S33, the resting cells could degrade 98 center dot 87% of nicotine in tobacco solid waste with the concentration as 30 mg nicotine g(-1) dry weight tobacco solid waste within 7 h at a maximal rate of 0 center dot 46 g nicotine h(-1) g(-1) dry cell.Conclusions:This is the first report that Agrobacterium sp. has the ability to degrade nicotine. Agrobacterium sp. S33 could use nicotine as the sole source of carbon and nitrogen. The use of resting cells of the strain S33 prepared from the nicotine-glucose-ammonium medium was an effective method to degrade nicotine and detoxify tobacco solid waste.Significance and Impact of the Study:Nicotine in tobacco wastes is both toxic and harmful to human health and the environment. This study showed that Agrobacterium sp. S33 may be suitable for the disposal of tobacco wastes and reducing the nicotine content in tobacco leaves.