Isolation of Coralmycins A and B, Potent Anti-Gram Negative Compounds from the Myxobacteria Corallococcus coralloides M23
Isolation of Coralmycins A and B, Potent Anti-Gram Negative Compounds from the Myxobacteria Corallococcus coralloides M23
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DOI：
10.1021/acs.jnatprod.6b00294
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发表时间：
2016-09-01
影响因子：
5.1
通讯作者：
Kim, Won-Gon
中科院分区：
文献类型：
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作者：
Kim, Yu Jin;Kim, Hyun-Ju;Kim, Won-Gon
从粘细菌珊瑚球菌 M23 的培养物中分离出两种新的强效抗革兰氏阴性化合物珊瑚霉素 A (1) 和 B (2)，以及另一种衍生物 (3)，后者被鉴定为最近报道的cystobactamid 919-2。通过光谱、旋光度和 CD 数据的解释阐明了它们的结构，包括相对立体化学。 3的相对立体化学被修改为“S*R*”NMR分析。 1 对革兰氏阴性病原体的抗菌活性最强，包括大肠杆菌、铜绿假单胞菌、鲍曼不动杆菌和肺炎克雷伯菌。 MIC 为 0.1-4 μg/mL；这些 MIC 分别比 3 和 2 的抗菌活性强 4-10 和 40-100 倍。因此，这些数据表明β-甲氧基天冬酰胺单元和苯甲酸单元的羟基对于抗菌活性至关重要。
Two new potent anti-Gram negative compounds, coralmycins A (1) and B (2), were isolated from cultures of the myxobacteria Corallococcus coralloides M23, together with another derivative (3) that was identified as the very recently reported cystobactamid 919-2. Their structures including the relative stereochemistry were elucidated by interpretation of spectroscopic, optical rotation, and CD data. The relative stereochemistry of 3 was revised to "S*R*" NMR analysis. The antibacterial activity of 1 was most potent against Gram-negative pathogens, including Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumanii, and Klebsiella pneumoniae. with MICs of 0.1-4 mu g/mL; these MICs were 4-10 and 40-100 times stronger than the antibacterial activities of 3 and 2, respectively. Thus, these data indicated that the beta-methoxyasparagine unit and the hydroxy group of the benzoic acid unit were critical for antibacterial activity.