Selective inhibition of ammonia oxidising archaea by simvastatin stimulates growth of ammonia oxidising bacteria
Selective inhibition of ammonia oxidising archaea by simvastatin stimulates growth of ammonia oxidising bacteria
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DOI：
10.1016/j.soilbio.2019.107673
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发表时间：
2020-02
影响因子：
9.7
通讯作者：
Jun Zhao;M. Bello;Yiyu Meng;J. Prosser;C. Gubry-Rangin
中科院分区：
文献类型：
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作者：
Jun Zhao;M. Bello;Yiyu Meng;J. Prosser;C. Gubry-Rangin
了解和区分土壤硝化中氨氧化古菌（AOA）和氨氧化细菌（AOB）的相对生长和活性的愿望增加了对这两组的选择性抑制剂的寻找。本研究旨在研究辛伐他汀作为纯培养物和土壤中的特异性 AOA 抑制剂的效力和特异性，并确定 AOA 抑制对氨氧化活性和 AOB 生长的影响，假设当消除 AOA 的 NH4+ 竞争时，AOB 生长会更高。辛伐他汀在浓度为 8–100 μM 时选择性抑制所有测试 AOA 的纯培养物。在低和高 NH4+ 浓度培养 21 天的土壤微观世界中，辛伐他汀在酸性（pH 4.5）和近中性（pH 6.5）土壤中选择性抑制 AOA 而不是 AOB。此外，辛伐他汀抑制AOA后，在两种NH4+浓度下AOB的生长均显着增加，这表明AOA和AOB之间对底物的竞争是限制NH4+限制土壤中AOB生长的关键因素。因此辛伐他汀可以作为选择性AOA抑制剂来研究土壤中AOB的动力学特征以及复杂环境中AOA与AOB之间的竞争。
The desire to understand and distinguish the relative growth and activity of ammonia oxidising archaea (AOA) and ammonia oxidising bacteria (AOB) in soil nitrification has increased the search for selective inhibitors of these two groups. This study aimed to investigate the potency and specificity of simvastatin as a specific AOA inhibitor in pure cultures and in soil and to determine the effect of AOA inhibition on both ammonia oxidation activity and growth of AOB, under the hypothesis that AOB growth is higher when competition for NH4+from AOA is removed. Simvastatin selectively inhibited pure cultures of all tested AOA at concentrations of 8–100 μM. In soil microcosms incubated for 21 days with low and high NH4+concentrations, AOA but not AOB were selectively inhibited by simvastatin in both acidic (pH 4.5) and near-neutral (pH 6.5) soils. Additionally, growth of AOB significantly increased at both NH4+concentrations following inhibition of AOA by simvastatin, suggesting that competition for substrate between AOA and AOB is a key factor restraining AOB growth in NH4+-limited soils. Simvastatin can therefore be used as a selective AOA inhibitor to investigate kinetic characteristics of AOB in soils and to study competition between AOA and AOB in complex environments.