Comparisons of two polymorphic species of Ostertagia and phylogenetic relationships within the Ostertagiinae (Nematoda: Trichostrongyloidea) inferred from ribosomal DNA repeat and mitochondrial DNA sequences.
Comparisons of two polymorphic species of Ostertagia and phylogenetic relationships within the Ostertagiinae (Nematoda: Trichostrongyloidea) inferred from ribosomal DNA repeat and mitochondrial DNA sequences.
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从核糖体 DNA 重复和线粒体 DNA 序列推断 Ostertagia 的两个多态性物种和 Ostertagiinae（线虫纲：毛圆线虫总科）内的系统发育关系的比较。
DOI：
10.2307/3284592
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
J. Lichtenfels
中科院分区：
文献类型：
--
作者：
D. Zarlenga;E. Hoberg;F. Stringfellow;J. Lichtenfels
利用酶切技术从6种奥斯特线虫亚科（Ostertagiinae）、捻转血矛线虫（Haemonchus contortus）和普氏血矛线虫（Haemonchus placei）中扩增出第一内转录间隔区（ITS-1）（rDNA）和线粒体（mt）DNA来源的细胞色素氧化酶I基因（考克斯-1），并进行克隆和序列测定。分析的考克斯-1基因的一部分是393个碱基对（bp）的长度和包含33种内的多态性碱基变化在28个同义位点。ITS-1 rDNA共有序列范围为392 bp（奥斯特线虫Ostertagia ostertagi/琴琴奥斯特线虫Ostertagia lyrata）和404 bp（环纹海鲶H. contortus、扭叶隐翅虫H.地点i）。这些数据都被用于距离分析，以评估的概念内的多态性种属奥斯特线虫和简约分析，以评估系统发育关系内有限的一组奥斯特线虫亚科。ITS-1和考克斯-1数据的成对相似性得分显示，建议的二型种奥斯特线虫之间的保守位点的数量最多。相似性水平在考克斯-1数据中较低，这是由于大量的同义碱基变化。同样的数据的最大简约分析没有反驳O。奥斯特塔吉岛lyrata和Ostertagia mossil/Ostertagia dikmansi为二型种，并支持这些奥斯特线虫相对于血吸虫外群代表的单系性。在ITS-1 rDNA数据的单一最简约的树，奥斯特线虫属的一个亚支。包括典型地出现在鹿科宿主中的具有平行合突和长食管瓣的形式。
The first internal transcribed spacer DNA (ITS-1) (rDNA) and the mitochondrial (mt) DNA-derived cytochrome oxidase I gene (COX-1) were enzymatically amplified, cloned and sequenced from 6 nominal species of Ostertagiinae as well as Haemonchus contortus and Haemonchus placei. The portion of the COX-1 gene analyzed was 393 base pairs (bp) in length and contained 33 within species polymorphic base changes at 28 synonymous sites. The ITS-1 rDNA consensus sequences ranged from 392 bp (Ostertagia ostertagi/Ostertagia lyrata, Teladorsagia circumcincta) to 404 bp (H. contortus, H. placei). These data were used both in a distance analysis to assess the concept of polymorphic species within the genus Ostertagia and in parsimony analysis to assess phylogenetic relationships within a limited group of Ostertagiinae. Pairwise similarity scores of both ITS-1 and COX-1 data showed the highest number of conserved sites between the proposed dimorphic species of Ostertagia. The level of similarity was lower in the COX-1 data due to the high number of synonymous base changes. Analysis by maximum parsimony of the same data did not refute O. ostertagi/O. lyrata and Ostertagia mossil/Ostertagia dikmansi as dimorphic species and supported monophyly of these ostertagiines relative to representatives of the haemonchine outgroup. In the single most parsimonious tree from ITS-1 rDNA data, a subclade of Ostertagia spp. included forms possessing parallel synlophes and long esophageal valves that typically occur in cervid hosts.