Cell survival in spheroids irradiated with heavy-ion beams.
Cell survival in spheroids irradiated with heavy-ion beams.
复制标题
重离子束照射的球体中的细胞存活率。
作者：
A. Rodriguez;E. Alpen
已经用X射线和高能碳离子和霓离子束进行了研究，以评估高LET细胞的RBE和细胞间通讯的可能贡献。比较了以多细胞球体形式照射的细胞和悬浮液照射的细胞的照射后存活特性。与225kVPx射线、400 MeV/amu碳离子和425 MeV/amu霓离子进行了比较。球形细胞的X-射线细胞存活曲线的D/sub0/‘S大于单细胞悬液中细胞的存活曲线。比较扩展的Bragg峰的近、中、远端区域对球体中细胞杀伤的有效性，表明细胞失活随着LET的增加而增加。中远峰区的存活剂量关系呈指数关系，10%存活率时的RBE分别为1.9和2.1，而近峰区的RBE为1.5。对霓虹峰扩展布拉格峰不同区域的存活数据的分析表明，中峰的最大RBE为10%，存活率为2.5，而在远峰照射的最低RBE值为1.6。我们得出结论，在霓虹束的远峰区域，较高的平均LET(200keV/.×..m)超过了最大有效LET，对于这种离子来说，LET可能低于此值。
Studies have been carried out with x rays and high-energy carbon and neon ion beams to evaluate high-LET cell survival in terms of RBE and possible contributions of intercellular communication. Comparisons were made of the postirradiation survival characteristics for cells irradiated as multicellular spheroids and for cells irradiated in suspension. Comparison is made with 225-kVp x rays, 400 MeV/amu carbon ions, and 425 MeV/amu neon ions. X-ray cell survival curves for cells from spheroids had larger D/sub 0/'s than did the survival curve for cells grown in single-cell suspension. Comparison of the effectiveness of the proximal, mid, and distal regions of the spread-out Bragg peak of carbon ions for cell killing in spheroids indicated that cell inactivation increased as a function of increasing LET. The survival dose relationship in the mid and distal peak regions was exponential, and the RBE, at 10% survival, was 1.9 and 2.1 compared to 1.5 for the proximal peak. Examination of survival data for various regions in the spread Bragg peak of neon showed that a maximum RBE at 10% survival of 2.5 was found for the midpeak, while the lowest RBE value of 1.6 for neon was found for irradiation in the distal peak.more » We conclude that the higher average LET (200 keV/..mu..m) in the distal peak region of the neon beam exceeds the maximally effective LET, which for this ion is probably below this value.« less