Rational synthesis of 1,3-diolein by enzymatic esterification
Rational synthesis of 1,3-diolein by enzymatic esterification
复制标题
DOI：
10.1016/j.jbiotec.2012.02.006
复制
发表时间：
2012-05-31
影响因子：
4.1
通讯作者：
Liu, De-Hua
中科院分区：
文献类型：
--
作者：
Duan, Zhang-Qun;Du, Wei;Liu, De-Hua
本课题组已经证实，叔丁醇是酶促油酸和甘油酯化制备1，3-双油酯的优良反应介质。为了实现1，3-二联烯的合理合成，采用响应面方法考察了各重要变量及其交互作用对产物合成的影响。在最佳反应条件下(62.4℃，0.75g Novozym 435，底物摩尔比(油酸/甘油)2.4，4.8g叔丁醇)，双甘油酯的产率可达87.4%，1，3-双甘油烯/双甘油酯的比值高达87.8%，与预测值非常接近。与之前的单因素实验结果相比，虽然双甘油酯的产率和1，3-双甘油烯/双甘油酯的比值没有明显提高，但加酶量和反应介质分别节省了25%和20%，这是对酶法工艺的显著改进。(C)2012爱思唯尔B.V.保留所有权利。
t-Butanol was an excellent reaction medium for enzyme-mediated esterification of oleic acid with glycerol for 1,3-diolein preparation which has been proved by our group. Herein, to achieve the rational synthesis of 1,3-diolien, response surface methodology was applied to examine the effects of the significant variables and their reciprocal effects on the product synthesis. Under the optimal conditions (62.4 degrees C, 0.75 g Novozym 435, substrate molar ratio (oleic acid/glycerol) 2.4 and 4.8 g t-butanol), the diolein yield of 87.4% could be achieved, and the value of 1,3-diolien/diolein was as high as 87.8%, which were quite close to the predicted values. Compared with the results of our previous single factor experiment, although the values of diolein yield and 1,3-diolien/diolein could not be improved markedly, the enzyme dosage and the reaction medium were spared by 25% and 20%, respectively, which was a remarkable improvement of the enzymatic process. (C) 2012 Elsevier B.V. All rights reserved.