PROPERTIES OF A CRYPTIC HIGH-FREQUENCY TRANSDUCING PHAGE IN STAPHYLOCOCCUS AUREUS
PROPERTIES OF A CRYPTIC HIGH-FREQUENCY TRANSDUCING PHAGE IN STAPHYLOCOCCUS AUREUS
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DOI：
10.1016/0042-6822(67)90105-5
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发表时间：
1967-01-01
期刊：
影响因子：
3.7
通讯作者：
NOVICK, R
中科院分区：
文献类型：
--
作者：
NOVICK, R
红霉素抗性的高频转导元件。该元件P11 de显然是温和噬菌体P11和青霉素酶质粒γ之间重组事件的结果;它缺乏质粒和噬菌体的实质部分。噬菌体部分是隐蔽的，既不赋予溶原性也不赋予携带它的宿主细胞的超感染免疫力;辅助噬菌体是生产转导颗粒所必需的，但不是转导过程所必需的。可证明的噬菌体相关性质包括UV灭活的同源噬菌体的再活化和温度敏感性噬菌体突变体的拯救。胞质部分包括红霉素抗性标记，而负责质粒相容性的mc区参与特异性宿主-质粒相互作用并为质粒复制所需;所有其他已知的质粒标记均缺失。Pllde的自主复制以及其相对于前噬菌体和其他质粒的细胞内行为似乎受其mc决定簇支配。在这些方面，Pllde类似于亲本γ质粒。然而，这种质粒控制被活性Pll取代，其诱导Pllde广泛繁殖。Pllde可以与野生型质粒重组;所得重组体不以高频率转导，也不重新激活UV灭活的噬菌体颗粒。基于这些发现，一个可能的遗传结构P11 de的，并与自然发生的质粒的地图进行比较。
A high-frequency transducing element for erythromycin resistance in S. aureus was found. This element, P11de, is apparently the result of a recombinational event between a temperate phage, P11, and a penicillinase plasmid, gamma; it lacks substantial sections both of the plasmid and of the bacteriophage. The phage moiety is cryptic, conferring neither lysogeny nor superinfection immunity upon host cells carrying it; helper phage is required for the production of transducing particles but not for the transduction process. Demonstrable phage-related properties include reactivation of UV -inactivated homologous phage and rescue of temperature-sensitive phage mutants. The plasmic moiety includes an erythromycin resistance marker, and the mc region, which is responsible for plasmid compatibility, is involved in a specific host-plasmid interaction and is required for plasmid replication; all the other known plasmid markers are missing. The autonomous replication of Pllde as well as its intracellular behavior vis-a-vis prophages and other plasmids appear to be governed by its mc determinant. In these respects Pllde is similar to the parental gamma plasmid. This plasmid control, however, is superseded by active Pll, which induces Pllde to multiply extensively. Pllde can recombine with wild-type plasmids; the resulting recombinants are not transduced at high frequency, nor do they reactivate UV inactivated phage particles. Based on these findings, a possible genetic structure for P11de is presented and compared with maps of naturally occurring plasmids.