Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene.
Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene.
复制标题
纳米2基因的CRISPR/CAS9编辑生成种系的雄性猪。
DOI：
10.1038/srep40176
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发表时间：
2017-01-10
影响因子：
4.6
通讯作者：
Oatley JM
中科院分区：
文献类型：
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作者：
Park KE;Kaucher AV;Powell A;Waqas MS;Sandmaier SE;Oatley MJ;Park CH;Tibary A;Donovan DM;Blomberg LA;Lillico SG;Whitelaw CB;Mileham A;Telugu BP;Oatley JM
基因组编辑工具已经彻底改变了包括家畜在内的转基因动物的产生。特别地，家猪是经证实的人类生理学模型和农业上重要的物种。在这项研究中，我们利用CRISPR/Cas9系统编辑猪胚胎中的NANOS 2基因，以产生具有单等位基因和双等位基因突变的后代。我们发现NANOS 2基因敲除的猪与表型敲除的小鼠相比具有雄性特异性生殖系消融，但睾丸发育的其他方面均正常。此外，具有一个完整NANOS 2等位基因的公猪和敲除母猪是可育的。从农业的角度来看，NANOS 2基因敲除的雄性猪有望成为移植供体精原干细胞的理想替代品，以扩大来自遗传上理想的父系的配子的可用性。
Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact NANOS2 allele and female knockout pigs are fertile. From an agriculture perspective, NANOS2 knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires.