Wavelength-scanning surface plasmon resonance imaging
Wavelength-scanning surface plasmon resonance imaging
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DOI：
10.1364/ao.44.003468
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发表时间：
2005-06-10
期刊：
影响因子：
1.9
通讯作者：
Wakida, S
中科院分区：
文献类型：
--
作者：
Otsuki, S;Tamada, K;Wakida, S
提出了一种新的表面等离子体共振（SPR）成像技术。在不同波长下进行测量后，在图像的每个像素处确定提供最小亮度的波长（SPR波长）。利用菲涅耳计算得到的非线性校准曲线，可以将SPR波长的二维映射转换为厚度分布。在空气中评估金膜上的蛋白质薄层阵列，以呈现纳米尺度的层表面结构。(c)2005年，美国光学学会。
A new surface plasmon resonance (SPR) imaging technique was proposed. After measurements were conducted at varying wavelengths, the wavelength affording the minimum brightness (SPR wavelength) was determined at each pixel of the image. A two-dimensional map of the SPR wavelength could be converted to a thickness profile by use of a nonlinear calibration curve, which was obtained by Fresnel calculation. An array of protein thin layers on a gold film was evaluated in air to present the layers' surface structure in nanometer scale. (c) 2005 Optical Society of America.