Challenges in DNA motion control and sequence readout using nanopore devices.
Challenges in DNA motion control and sequence readout using nanopore devices.
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DOI：
10.1088/0957-4484/26/7/074004
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发表时间：
2015-02-20
期刊：
影响因子：
3.5
通讯作者：
Wanunu M
中科院分区：
文献类型：
--
作者：
Carson S;Wanunu M
纳米孔被誉为潜在的下一代DNA测序仪，可以提供廉价，高通量的DNA分析。在这篇评论中，我们提出了一个详细的总结，正在调查的各种传感技术用于DNA测序和绘图应用。纳米孔作为可靠的DNA分析工具的成功的关键僵局是DNA易位的快速和随机性质。我们讨论了生物马达的掺入，通过一个孔基地基地的基地，以及许多实验性的修改，试图减缓和控制DNA运输的目的。
Nanopores are being hailed as a potential next-generation DNA sequencer that could provide cheap, high-throughput DNA analysis. In this review we present a detailed summary of the various sensing techniques being investigated for use in DNA sequencing and mapping applications. A crucial impasse to the success of nanopores as a reliable DNA analysis tool is the fast and stochastic nature of DNA translocation. We discuss the incorporation of biological motors to step DNA through a pore base-by-base, as well as the many experimental modifications attempted for the purpose of slowing and controlling DNA transport.