A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species
A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species
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拟盘拟盘属的多位点骨干树，具有 14 个新物种的多相特征
DOI：
10.1007/s13225-012-0198-1
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发表时间：
2012-09-01
期刊：
影响因子：
20.3
通讯作者：
Hyde, Kevin D.
中科院分区：
文献类型：
--
作者：
Maharachchikumbura, Sajeewa S. N.;Guo, Liang-Dong;Hyde, Kevin D.
拟盘拟盘属是一个分类学混乱、致病性和化学创造性的属，需要使用基于前型和前表型培养物的多基因系统发育进行严格的重新检查。在本研究中，通过形态学和 ITS、β-微管蛋白和 tef1 基因序列数据分析，对 40 个拟盘拟盘菌分离株（包括从中国各种寄主植物的活体和死体植物材料中收集的 28 个菌株）进行了研究。根据分子和形态学数据，我们描述了 14 个新种（Pestalotiopsis asiatica、P. chinensis、P. chrysea、P. clavata、P. diversiseta、P. ellipsospora、P. inflexa、P. intermedia、P. Linearis、P. Rosea、P. saprophyta、P. umberspora、P. unicolor 和 P. verruculosa），其中 3 个种为表型化（P. adusta、P. clavispora 和 P. foedans）。在用于解析隐性拟盘菌属物种的 10 个基因区域（ACT、β-微管蛋白、CAL、GPDH、GS、ITS、LSU、RPB 1、SSU 和 tef1）中，ITS、β-微管蛋白和 tef1 被证明是更好的标记。其他基因区域的用处较小，因为 PCR 扩增和/或解决物种边界的能力较差。作为单基因，tef1 满足理想候选基因的要求，并且由于其更好的物种分辨率和 PCR 成功而在物种定界中发挥良好作用。尽管 β-微管蛋白在物种间显示出相当好的差异，但与单基因分析相比，ITS、β-微管蛋白和 tef1 基因数据的组合提供了最佳分辨率。
Pestalotiopsis is a taxonomically confused, pathogenic and chemically creative genus requiring a critical re-examination using a multi-gene phylogeny based on ex-type and ex-epitype cultures. In this study 40 isolates of Pestalotiopsis, comprised of 28 strains collected from living and dead plant material of various host plants from China were studied by means of morphology and analysis of ITS, β–tubulin and tef1 gene sequence data. Based on molecular and morphological data we describe 14 new species (Pestalotiopsis asiatica, P. chinensis, P. chrysea, P. clavata, P. diversiseta, P. ellipsospora, P. inflexa, P. intermedia, P. linearis, P. rosea, P. saprophyta, P. umberspora, P. unicolor and P. verruculosa) and three species are epitypified (P. adusta, P. clavispora and P. foedans). Of the 10 gene regions (ACT, β-tubulin, CAL, GPDH, GS, ITS, LSU, RPB 1, SSU and tef1) utilized to resolve cryptic Pestalotiopsis species, ITS, β–tubulin and tef1 proved to be the better markers. The other gene regions were less useful due to poor success in PCR amplification and/or in their ability to resolve species boundaries. As a single gene tef1 met the requirements for an ideal candidate and functions well for species delimitation due to its better species resolution and PCR success. Although β-tubulin showed fairly good differences among species, a combination of ITS, β-tubulin and tef1 gene data gave the best resolution as compared to single gene analysis.