General control non-repressible 20 functions in the salt stress response of Arabidopsis seedling by modulating ABA accumulation
General control non-repressible 20 functions in the salt stress response of Arabidopsis seedling by modulating ABA accumulation
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DOI：
10.1016/j.envexpbot.2022.104856
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发表时间：
2022-03-26
影响因子：
5.7
通讯作者：
Lu, Ying-Tang
中科院分区：
文献类型：
--
作者：
Ding, Feng;Zhang, Bing-Lei;Lu, Ying-Tang
虽然大多数ABC蛋白是转运蛋白，但ABC F亚家族的蛋白缺乏所需的跨膜结构域，因此，它们的功能知之甚少。在这里，我们报告说，F亚家族成员一般控制非阻遏20（GCN 20）介导阿坝和活性氧在植物盐胁迫过程中积累。我们证明盐条件诱导GCN 20的表达。阿坝积累量高的突变体gCN 20 -1对高盐胁迫的敏感性高于野生型，表现为种子发芽率和鲜重的降低，阿坝积累量的降低可以挽救突变体降低的耐盐性，表明阿坝参与了GCN 20介导的盐胁迫响应。盐胁迫促进了gcn 20 -1中一些与胁迫相关的基因和阿坝信号响应基因的表达。此外，当经受盐胁迫时，gcn 20 -1植物具有较少的CAT和SOD活性，并且因此具有比野生型植物更多的ROS。这些结果表明，GCN 20通过调节阿坝的积累，密切参与了高盐胁迫引起的响应。
Although most ABC proteins are transporters, proteins of the ABC F subfamily lack the required transmembrane domains and, consequently, their functions are poorly understood. Here, we report that the F subfamily member general control non-repressible 20 (GCN20) mediates ABA and ROS accumulation in plants during salt stress. We demonstrate that salt conditions induce the expression of GCN20. The gcn20-1 mutant with more ABA accumulation is more sensitive to high salinity than the wild type in terms of decreased seed germination and fresh weight, and decreasing ABA accumulation can rescue reduced salt tolerance of the mutant, revealing the involvement of ABA in GCN20-mediated salt stress response. Consistently, salt stress promotes a number of stress-related and ABA signal-responsive genes expression in the gcn20-1 plants. In addition, the gcn20-1 plants have less CAT and SOD activity, and thus more ROS than wild-type plants when subjected to salt stress. These findings display that GCN20 is closely involved in the stress response caused by high salinity through modulation of ABA accumulation.