Inkjet printing Schwann cells and neuronal analogue NG108-15 cells
Inkjet printing Schwann cells and neuronal analogue NG108-15 cells
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DOI：
10.1088/1758-5090/8/1/015017
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发表时间：
2016-03
期刊：
影响因子：
9
通讯作者：
C. Tse;R. Whiteley;Tong Yu;J. Stringer;S. MacNeil;J. Haycock;Patrick J. Smith
中科院分区：
文献类型：
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作者：
C. Tse;R. Whiteley;Tong Yu;J. Stringer;S. MacNeil;J. Haycock;Patrick J. Smith
采用直径为60 μm的压电喷墨打印机，在70-230 V范围内打印猪雪旺细胞和神经元模拟物NG108-15细胞，并对打印后的细胞活力和质量进行分析。打印后立即检测到>的86%和>的90%的神经元和胶质细胞的存活率，并没有观察到施加电压与细胞存活率的相关性。与对照相比，打印的神经元细胞更早地产生神经突，并且在几天后，产生更长的神经突，这在第7天变得最明显。到第7天，神经突的数量也变得相似，细胞的增殖能力与未打印的细胞（对照组）相似。这种喷墨打印细胞的方法为研究神经元-胶质细胞相互作用提供了一个技术平台，与标准细胞播种相比，细胞活力没有显著差异。这些技术可以用于芯片实验室技术，并为神经科学应用创建打印神经网络。
Porcine Schwann cells and neuronal analogue NG108-15 cells were printed using a piezoelectric-inkjet-printer with a nozzle diameter of 60 μm, within the range of 70–230 V, with analysis of viability and quality after printing. Neuronal and glial cell viabilities of >86% and >90% were detected immediately after printing and no correlation between voltage applied and cell viability could be seen. Printed neuronal cells were shown to produce neurites earlier compared to controls, and over several days, produced longer neurites which become most evident by day 7. The number of neurites becomes similar by day 7 also, and cells proliferate with a similar viability to that of non-printed cells (controls). This method of inkjet printing cells provides a technical platform for investigating neuron–glial cell interactions with no significant difference to cell viability than standard cell seeding. Such techniques can be utilized for lab-on-a-chip technologies and to create printed neural networks for neuroscience applications.