CHROMATIN STRUCTURE AND CELL CYCLE
CHROMATIN STRUCTURE AND CELL CYCLE
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DOI：
10.1073/pnas.69.8.2224
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发表时间：
1972-01-01
影响因子：
11.1
通讯作者：
PEDERSON, T
中科院分区：
文献类型：
--
作者：
PEDERSON, T
胰腺 DNase I 用于探测从同步 HeLa 细胞中分离的染色质结构。在细胞周期的 G1、S 和 G2 期，染色质中的 DNA 免受 DNase 攻击的程度有所不同。此外，接触抑制的非洲绿猴肾细胞染色质的 DNase 敏感性与活跃分裂、亚融合培养物的不同。在测试的所有酶浓度（5000 倍范围）和孵育时间（15 分钟至 2 小时）下，一致地观察到这些细胞周期依赖性染色质变化。结果表明，DNA 和染色体蛋白之间的复合程度在间期发生变化，并且表明可见有丝分裂的染色体卷曲周期可能以更微妙的形式延伸到整个细胞周期。
Pancreatic DNase I is used to probe the structure of chromatin isolated from synchronized HeLa cells. The degree to which DNA in chromatin is protected from DNase attack varies during the G1, S, and G2phases of the cell cycle. In addition, the DNase sensitivity of chromatin from contact-inhibited African green monkey kidney cells differs from that of actively dividing, subconfluent cultures. These cell cycle-dependent chromatin changes were observed consistently at all enzyme concentrations (5000-fold range) and incubation times (15 min-2 hr) tested. The results indicate that the degree of complexing between DNA and chromosomal proteins changes during interphase, and they suggest that the chromosome coiling cycle of visible mitosis may extend in more subtle form over the entire cell cycle.