Histone deacetylation is required for progression through mitosis in tobacco cells
Histone deacetylation is required for progression through mitosis in tobacco cells
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DOI：
10.1111/j.1365-313x.2004.02301.x
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发表时间：
2005-02-01
期刊：
影响因子：
7.2
通讯作者：
Grafi, G
中科院分区：
文献类型：
--
作者：
Li, Y;Butenko, Y;Grafi, G
核心组蛋白的翻译后修饰在染色质结构和功能中起着关键作用。在这里，我们研究组蛋白的翻译后修饰过程中的原生质体来自烟草叶肉细胞重新进入细胞周期，并通过有丝分裂的进展，评估其意义。组蛋白H3在赖氨酸残基4和9的甲基化在细胞周期的所有阶段中持续存在于染色体中。然而，乙酰化的H4和H3显着减少有丝分裂过程中的阶段特异性的方式，而组蛋白H4的脱乙酰化开始在前期和持续到末期，组蛋白H3保持乙酰化到中期，但在后期和末期脱乙酰化。组蛋白H3在丝氨酸10处的磷酸化在前期开始，伴随着组蛋白H4的去乙酰化，并持续到末期。阻止组蛋白去乙酰化酶抑制剂抑制素A（TSA）的组蛋白去乙酰化，导致在中期后期的原生质体的积累，并减少S10磷酸化在后期和末期，在培养的烟草细胞，TSA显着降低有丝分裂的数字的频率。我们的研究结果表明，组蛋白H4和H3在烟草原生质体中的脱乙酰化发生在有丝分裂过程中的阶段特异性的方式，并通过有丝分裂的进展是重要的。
Post-translational modifications of core histone proteins play a key role in chromatin structure and function. Here, we study histone post-translational modifications during reentry of protoplasts derived from tobacco mesophyll cells into the cell cycle and evaluate their significance for progression through mitosis. Methylation of histone H3 at lysine residues 4 and 9 persisted in chromosomes during all phases of the cell cycle. However, acetylation of H4 and H3 was dramatically reduced during mitosis in a stage-specific manner; while deacetylation of histone H4 commenced at prophase and persisted up to telophase, histone H3 remained acetylated up to metaphase but was deacetylated at anaphase and telophase. Phosphorylation of histone H3 at serine 10 was initiated at prophase, concomitantly with deacetylation of histone H4, and persisted up to telophase. Preventing histone deacetylation by the histone deacetylase inhibitor trichostatin A (TSA) led to accumulation of protoplasts at metaphase-anaphase, and reduced S10 phosphorylation during anaphase and telophase; in cultured tobacco cells, TSA significantly reduced the frequency of mitotic figures. Our results indicate that deacetylation of histone H4 and H3 in tobacco protoplasts occurs during mitosis in a phase-specific manner, and is important for progression through mitosis.