The conserved Nup107-160 complex is critical for nuclear pore complex assembly
The conserved Nup107-160 complex is critical for nuclear pore complex assembly
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DOI：
10.1016/s0092-8674(03)00235-6
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发表时间：
2003-04-18
期刊：
影响因子：
64.5
通讯作者：
Doye, V
中科院分区：
文献类型：
--
作者：
Walther, TC;Alves, A;Doye, V
核孔复合体 (NPC) 是大型多蛋白组装体，允许细胞质和细胞核之间的交通。在高等真核生物的有丝分裂过程中，核膜 (NE) 会分解，NPC 会分解。人们对 NPC 在有丝分裂退出时如何重组并融入 NE 的了解甚少。我们展示了在此过程中保守的 Nup1107-160 复合物的功能。在 HeLa 细胞中通过 RNAi 体内部分耗尽 Nup133 或 Nup107 导致多种核孔蛋白水平降低，并降低 NE 中的 NPC 密度。对体外核组装反应中的整个 Nup1107-160 复合物进行免疫耗竭，产生具有连续 NE 但没有 NPC 的细胞核。仅当在闭合 NE 形成之前读取 Nup107-160 复合物时，该表型才是可逆的。耗尽还阻止了 FG 重复核孔蛋白与染色质的结合。我们提出了一个逐步模型，其中有丝分裂后 NPC 组装通过早期招募 Nup107-160 复合物在染色质上启动。
Nuclear pore complexes (NPCs) are large multiprotein assemblies that allow traffic between the cytoplasm and the nucleus. During mitosis in higher eukaryotes, the Nuclear Envelope (NE) breaks down and NPCs disassemble. How NPCs reassemble and incorporate into the NE upon mitotic exit is poorly understood. We demonstrate a function for the conserved Nup1107-160 complex in this process. Partial in vivo depletion of Nup133 or Nup107 via RNAi in HeLa cells resulted in reduced levels of multiple nucleoporins and decreased NPC density in the NE. Immunodepletion of the entire Nup1107-160 complex from in vitro nuclear assembly reactions produced nuclei with a continuous NE but no NPCs. This phenotype was reversible only if Nup107-160 complex was readded before closed NE formation. Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex.