Flexible CRISPR library construction using parallel oligonucleotide retrieval.
Flexible CRISPR library construction using parallel oligonucleotide retrieval.
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DOI：
10.1093/nar/gkx181
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发表时间：
2017-06-20
影响因子：
14.9
通讯作者：
Luo J
中科院分区：
文献类型：
--
作者：
Read A;Gao S;Batchelor E;Luo J
CRISPR/Cas9-based gene knockout libraries have emerged as a powerful tool for functional screens. We present here a set of pre-designed human and mouse sgRNA sequences that are optimized for both high on-target potency and low off-target effect. To maximize the chance of target gene inactivation, sgRNAs were curated to target both 5΄ constitutive exons and exons that encode conserved protein domains. We describe here a robust and cost-effective method to construct multiple small sized CRISPR library from a single oligo pool generated by array synthesis using parallel oligonucleotide retrieval. Together, these resources provide a convenient means for individual labs to generate customized CRISPR libraries of variable size and coverage depth for functional genomics application.
