Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transection
Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transection
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DOI：
10.1006/exnr.1996.0098
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发表时间：
1996-06-01
影响因子：
5.3
通讯作者：
Bresnahan, JC
中科院分区：
文献类型：
--
作者：
Basso, DM;Beattie, MS;Bresnahan, JC
损伤的重复性是脊髓损伤(SCI)实验模型的一个重要特征，因为它限制了运动和解剖学结果指标的可变性。最近，一种更灵敏的运动评定量表Basso，Beattie，and Bresna han Scale(BBB)被开发出来，但还没有在严重脊髓损伤完全横断的大鼠身上进行测试。使用纽约大学的装置，让大鼠在T10从6.25、12.5、25和50 mm的高度将一根10g的棒落到暴露的脊髓上。一组25 mm和50 mm脊髓损伤的大鼠随后接受脊髓横断(SCI+TX)，并仅与仅横断(TX)的大鼠进行比较，以确定恢复对下行系统的依赖。在7-9周的运动测试后，除了12.5vs25 mm和25vs50 mm组外，所有组之间通过病变中心的髓鞘染色横断面上测量的白质百分比都有显著差异。运动恢复以6.25 mm组最大，远低于50 mm组，且与病变中心保留的组织数量呈正相关(p<；0.0001)。血脑屏障评分敏感性足以区分最严重的脊髓损伤(50 Mm)和完全脊髓损伤(p<；0.01)之间的显著运动差异。脊髓损伤后横断导致运动评分下降，大鼠不能用后肢行走或支撑体重(P<；0.01)，这表明运动恢复依赖于备用的下行系统。脊髓损伤+TX组在旷场测试中HL运动频率显著高于TX组(P<；0.005)。脊髓损伤+TX组的运动评分也有高于TX组的趋势(p>；0.05)。因此，备用下行系统似乎可以改变节段性系统，从而比单独的脊髓系统产生更大的行为改善。(C)1996年学术出版社。
Injury reproducibility is an important characteristic of experimental models of spinal cord injuries (SCI) because it limits the variability in locomotor and anatomical outcome measures. Recently, a more sensitive locomotor rating scale, the Basso, Beattie, and Bresnahan scale (BBB), was developed but had not been tested on rats with severe SCI complete transection. Rats had a 10-g rod dropped from heights of 6.25, 12.5, 25, and 50 mm onto the exposed cord at T10 using the NYU device. A subset of rats with 25 and 50 mm SCI had subsequent spinal cord transection (SCI + TX) and mere compared to rats with transection only (TX) in order to ascertain the dependence of recovery on descending systems. After 7-9 weeks of locomotor testing, the percentage of white matter measured from myelin-stained cross sections through the lesion center was significantly different between all the groups with the exception of 12.5 vs 25 mm and 25 vs 50 mm groups. Locomotor recovery was greatest for the 6.25-mm group and least far the 50-mm group and was correlated positively to the amount of tissue sparing at the lesion center (p < 0.0001). BBB scale sensitivity was sufficient to discriminate significant locomotor differences between the most severe SCI (50 mm) and complete TX (p < 0.01). Transection following SCI resulted in a drop in locomotor scores and rats were unable to step or support weight with their hindlimbs (p < 0.01), suggesting that locomotor recovery depends on spared descending systems. The SCI + TX group had a significantly greater frequency of HL movements during open field testing than the TX group (p < 0.005). There was also a trend for the SCI + TX group to have higher locomotor scores than the TX group (p > 0.05). Thus, spared descending systems appear to modify segmental systems which produce greater behavioral improvements than isolated cord systems. (C) 1996 Academic Press, Inc.