Generation and characterization of a novel shoulder contracture mouse model
Generation and characterization of a novel shoulder contracture mouse model
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DOI：
10.1002/jor.22943
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发表时间：
2015-11-01
影响因子：
2.8
通讯作者：
Horiuchi, Keisuke
中科院分区：
文献类型：
--
作者：
Oki, Satoshi;Shirasawa, Hideyuki;Horiuchi, Keisuke
冻结肩是一种相对常见的疾病，导致严重的疼痛和僵硬的肩关节。虽然这种疾病是自限性的，但症状往往持续多年，导致严重残疾。最近使用人类标本和动物模型的研究表明，冻结肩组织中的基因表达模式发生了明显的变化，这表明可以通过控制可能参与冻结肩发展的基因来实现新的治疗干预。为了实现这一目标，迫切需要开发一种可靠的动物关节挛缩模型，在该模型中，可以通过基因打靶和转基因技术来操纵基因表达。在这里，我们描述了一种新的肩挛缩小鼠模型。我们发现，该模型模拟了人类冻结肩的临床表现，并概括了人类和其他大型动物模型中冻结肩和关节挛缩的基因表达模式和组织学变化。该模型具有高度可重复性，没有任何重大并发症。因此，本模型可以作为一个有用的工具，研究冻结肩病因学和确定其潜在的靶基因。(c)2015骨科研究学会。由威利期刊公司出版J Orthop Res 33：1732-1738，2015.
Frozen shoulder is a relatively common disorder that leads to severe pain and stiffness in the shoulder joint. Although this disorder is self-limiting in nature, the symptoms often persist for years, resulting in severe disability. Recent studies using human specimens and animal models have shown distinct changes in the gene expression patterns in frozen shoulder tissue, indicating that novel therapeutic intervention could be achieved by controlling the genes that are potentially involved in the development of frozen shoulder. To achieve this goal, it is imperative to develop a reliable animal joint contracture model in which gene expression can be manipulated by gene targeting and transgenic technologies. Here, we describe a novel shoulder contracture mouse model. We found that this model mimics the clinical presentation of human frozen shoulder and recapitulates the changes in the gene expression pattern and the histology of frozen shoulder and joint contracture in humans and other larger animal models. The model is highly reproducible, without any major complications. Therefore, the present model may serve as a useful tool for investigating frozen shoulder etiology and for identifying its potential target genes. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1732-1738, 2015.