Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs.
Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs.
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DOI：
10.1523/jneurosci.1042-04.2004
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发表时间：
2004-06-02
期刊：
影响因子：
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通讯作者：
Henley JM
中科院分区：
文献类型：
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作者：
Ashby MC;De La Rue SA;Ralph GS;Uney J;Collingridge GL;Henley JM
AMPA受体（AMPARs）在突触中受到动态调节，但其胞吐作用和胞吞作用的时间过程和位置尚不清楚。因此，我们使用黄道型氟标记谷氨酸受体2来实时观察AMPAR表面表达的变化。我们发现突触和突触外AMPARs对NMDA受体激活的反应非常不同；突触外ampar的快速内化先于突触ampar的延迟去除。
AMPA receptors (AMPARs) are dynamically regulated at synapses, but the time course and location of their exocytosis and endocytosis are not known. Therefore, we have used ecliptic pHluorin-tagged glutamate receptor 2 to visualize changes in AMPAR surface expression in real time. We show that synaptic and extrasynaptic AMPARs respond very differently to NMDA receptor activation; there is a rapid internalization of extrasynaptic AMPARs that precedes the delayed removal of synaptic AMPARs.