Prognostic significance of CD100 expression in soft tissue sarcoma
Prognostic significance of CD100 expression in soft tissue sarcoma
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DOI：
10.1002/cncr.22764
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发表时间：
2007-07-01
期刊：
影响因子：
6.2
通讯作者：
Aozasa, Katsuyuki
中科院分区：
文献类型：
--
作者：
Ch'ng, EweSeng;Tomita, Yasuhiko;Aozasa, Katsuyuki
背景CD 100是一种IV类脑信号蛋白，在体外促进肿瘤细胞的血管生成、侵袭性生长、增殖和抗凋亡。探讨CD 100在软组织肉瘤（STS）中的表达及其与临床病理因素的关系和预后意义。在来自81名患者的石蜡包埋切片上，用化学方法评价了具有局限性STS的患者中的CD 100表达水平，所述患者包括47名男性和34名女性，中位年龄为54岁。染色强度被分类为弱于（水平1）或等于淋巴细胞的染色强度，其中染色肿瘤细胞的比率< 10%（水平2）或染色肿瘤细胞的比率> 10%（水平3）。平行进行Ki-67染色。42个肿瘤（52%）具有1级CD 100表达，18个肿瘤（22%）具有2级CD 100表达，21个肿瘤（26%）具有3级CD 100表达。与CD 100表达水平为1的肿瘤相比，CD 100表达水平为2和3的肿瘤与较高的有丝分裂计数、细胞构成、坏死率和Ki-67标记指数（LI）显著相关。CD 100表达与其他特征无相关性。在3种水平的CD 100表达中，较高的表达水平与较差的总体和无病生存期相关。多因素分析显示，CD 100表达（1级和2级vs 3级）和肿瘤大小（5 cm）是总生存率的独立预测因素（P
BACKGROUND. CD100, a class IV semaphorin, promotes angiogenesis, invasive growth, proliferation, and antiapoptosis of cancer cells in vitro. The expression of CD100 in soft tissue sarcoma (STS) and its correlation with clinicopathologic factors and prognostic significance were evaluated.METHODS. Expression levels of CD100 in patients with localized STS were evaluated immunohistochemically on paraffin- embedded sections from 81 patients, including 47 men and 34 women with a median age of 54 years. Staining intensity was categorized into weaker than (level 1) or equal to that of lymphocytes with a rate of < 10% stained tumor cells (level 2) or > 10% stained tumor cells (level 3). Ki-67 staining was performed in parallel.RESULTS. Forty-two tumors (52%) had level 1 CD100 expression, 18 tumors (22%) had level 2 CDIOO expression, and 21 tumors (26%) had level 3 CD100 expression. Tumors that had level 2 and 3 CD100 expression were correlated significantly with higher mitotic count, cellularity, ratio of necrosis, and Ki-67 labeling index (LI) compared with tumors that had level 1 CD100 expression. There was no correlation between CD100 expression and other characteristics. Among the 3 levels of CD100 expression, higher expression levels were correlated with poorer overall and disease-free survival. Multivariate analysis revealed that CD100 expression (levels 1 and 2 vs level 3) and tumor size ( 5 cm) were independent prognosticators for overall survival (P