Application of cellulose and amylose arylcarbamates as chiral selectors in counter-current chromatography
Application of cellulose and amylose arylcarbamates as chiral selectors in counter-current chromatography
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DOI：
10.1016/j.chroma.2005.12.061
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发表时间：
2006-02-24
影响因子：
4.1
通讯作者：
Minguillón, C
中科院分区：
文献类型：
--
作者：
Pérez, E;Santos, MJ;Minguillón, C
本文研究了纤维素和直链淀粉-三（3，5-二甲基苯基氨基甲酸酯）作为手性选择剂在逆流色谱分离对映体中的应用。各种有机/水两相溶剂系统和8个外消旋分析物的酸性，中性和碱性的性质进行了测试，用于此目的。采用经典洗脱模式和pH区带精制置换条件。在甲基异丁基酮（MIBK）/水溶液和甲基叔丁基醚（MTBE）/水溶液中，吲哚洛尔和华法林分别实现了部分对映体分离。对于这两个外消旋体的对映体过量值从84%到97%，在最佳条件下测试实现。然而，对于普萘洛尔、萘普生和N-（3，5-二硝基苯甲酰基）-（+/-）-亮氨酸，CCC未实现分离，这些药物在HPLC中易于通过相同的选择剂分离。(c)2005 Elsevier B. V.保留所有权利。
Here we studied the applicability of cellulose and amylose tris(3,5-dimethylphenylcarbamate) as chiral selectors for the separation of enantiomers by counter-current chromatography (CCC). A variety of organic/aqueous biphasic solvent systems and eight racemic analytes of acidic, neutral, and basic nature were tested for this purpose. Classical elution mode and pH-zone-refining displacement conditions were used. Partial enantioseparation of pindolol and warfarin was achieved in methyl isobutyl ketone (MIBK)/aqueous solution and methyl tert-butyl ether (MTBE)/aqueous solution, respectively. For these two racemates enantiomeric excess values from 84% to 97% were achieved under the best conditions tested. However, resolution by CCC was not achieved for propranolol, naproxen and N-(3,5-dinitrobenzoyl)-(+/-)-leucine, easily separated in HPLC by the same selectors. (c) 2005 Elsevier B.V. All rights reserved.