Gene expression of 5-fluorouracil metabolic enzymes in primary gastric cancer: Correlation with drug sensitivity against 5-fluorouracil
Gene expression of 5-fluorouracil metabolic enzymes in primary gastric cancer: Correlation with drug sensitivity against 5-fluorouracil
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DOI：
10.1016/j.canlet.2007.01.006
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发表时间：
2007-07-18
期刊：
影响因子：
9.7
通讯作者：
Nakao, Akimasa
中科院分区：
文献类型：
--
作者：
Kodera, Yasuhiro;Ito, Seiji;Nakao, Akimasa
胸苷合成酶(TS)、二氢嘧啶脱氢酶(DPD)、胸苷磷酸化酶(TP)和转硫酸磷酸核糖转移酶(OPRT)是预测氟嘧啶类化疗疗效的指标。应用逆转录聚合酶链式反应(RT-PCR)定量检测93例胃癌石蜡包埋标本中TS、DPD、TP和OPRT基因的表达。对93例手术切除的原发灶进行体外药敏试验和组织培养药敏试验。OPRT基因的表达与肿瘤的部位、病理类型、临床分期等临床病理因素无明显相关性，但与5-FU的药物敏感性呈弱相关(R=0.219，p=0.0343)。OPRT被认为对药物敏感性有重大影响，尽管还不足以仅根据这种酶的mRNA表达来预测5FU的敏感性。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
Thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), and orotate phosphoribosyltransfierase (OPRT) have been reported to be predictive parameters for the efficacy of fluoropyrimidine-based chemotherapy. mRNA expression of TS, DPD, TP, and OPRT were quantified by reverse-transcriptase polymerase chain reaction after harvesting cancer cells from 93 paraffin-embedded specimens of gastric cancer through laser capture microdissection. In vitro chemosensitivity testing by histoculture drug response assay was performed with surgically resected primary lesions of the same 93 patients. No significant correlation was observed between the mRNA expression and location of the tumor, histopathologic type, clinical stage, and other clinicopathologic variables, with the exception that OPRT mRNA had a weak correlation with drug sensitivity against 5FU (R = 0.219, p = 0.0343). OPRT was considered to have a major impact on drug sensitivity, although not sufficiently so to enable prediction of 5FU sensitivity solely based on the mRNA expression of this enzyme. (c) 2007 Elsevier Ireland Ltd. All rights reserved.