DISTRIBUTION OF SUB-CELLULAR BIOLUMINESCENT SOURCES IN A DINOFLAGELLATE, PYROCYSTIS-FUSIFORMIS
DISTRIBUTION OF SUB-CELLULAR BIOLUMINESCENT SOURCES IN A DINOFLAGELLATE, PYROCYSTIS-FUSIFORMIS
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DOI：
10.2307/1540994
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发表时间：
1982-01-01
影响因子：
1.6
通讯作者：
CASE, JF
中科院分区：
文献类型：
--
作者：
WIDDER, EA;CASE, JF
梭梭棘在亚细胞生物发光源的分布和机械兴奋性方面表现出节律性变化。夜相细胞的生物发光可以通过机械或低pH值来刺激，并且起源于围绕中央大液泡的细胞质层中的微源。微光源是弱荧光，可能对应0.5 .mu。M或更小的细胞质内含物。随着白昼期的开始，生物发光变得机械地不兴奋，但对酸刺激有反应。微光源在早期从细胞外围消失，所有的发光都来自核周区和外围的微光源。在夜相开始之前，发光仍然是机械地不可激发的。在黑暗中维持的细胞在机械兴奋性、核周发光的发育和消失方面表现出相同的节律性变化，只是早期细胞的微光源在没有光诱导期的情况下不会从外围消失。提出了生物发光分布和机械兴奋性节律性变化的机制。
P. fusiformis exhibits rhythmic changes in the distribution and mechanical excitability of subcellular bioluminescent sources. Bioluminescence in night-phase cells can be stimulated either mechanically or by low pH and originates from microsources found throughout the cytoplasmic layer surrounding the large central vacuole. Microsources are weakly fluorescent and probably correspond to 0.5 .mu.m or smaller cytoplasmic inclusions. With the onset of day phase, bioluminescence becomes mechanically inexcitable but responds to acid stimulation. Microsources disappear from the cell periphery during early day phase and all luminescence originates from the perinuclear region and from microsources in the periphery. Luminescence remains mechanically inexcitable until the onset of night phase. Cells maintained in darkness exhibit the same rhythmic changes in mechanical excitability, development and disappearance of the perinuclear luminescence, except that microsources do not disappear from the periphery of early day-phase cells without a light induction period. Mechanisms which might underlie the rhythmic changes in bioluminescence distribution and mechanical excitability are proposed.