The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen.
The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen.
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DOI：
10.1083/jcb.107.3.841
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发表时间：
1988-09
期刊：
影响因子：
--
通讯作者：
Richardson WD
中科院分区：
文献类型：
--
作者：
Dingwall C;Robbins J;Dilworth SM;Roberts B;Richardson WD
核纤溶蛋白的羧基末端尾部，指定进入细胞核，包含四个短序列，与先前鉴定的核定位序列相似。我们发现这些都不能将鸡肌丙酮酸激酶定位到细胞核。缺失分析用于确定核定位序列的极限，并表明14个氨基酸片段（RPAATKKAGQAKKK）应作为最小核定位序列。然而，当直接测试时，该序列无法将丙酮酸激酶定位到细胞核。恢复该序列两端核纤溶蛋白序列的三个氨基酸，产生了能够定位丙酮酸激酶到细胞核的序列。在功能序列AVKRPAATKKAGQAKKK、rpaatkkagqakkkld和两端有附加氨基酸的序列AVKRPAATKKAGQAKKKKLD中存在14个氨基酸的最小核定位序列。因此，最小序列元件是必需的，但不足以运输到细胞核。核纤溶蛋白核定位序列的这种不同寻常的特征表明它可能与核转运机制相互作用。
The carboxy-terminal tail of nucleoplasmin, which specifies entry into the cell nucleus, contains four short sequences that are similar to previously identified nuclear location sequences. We show that none of these is able to locate chicken muscle pyruvate kinase to the cell nucleus. Deletion analysis was used to determine the limits of a nuclear location sequence and indicated that a 14-amino acid segment (RPAATKKAGQAKKK) should function as a minimal nuclear location sequence. When tested directly, however, this sequence was unable to locate pyruvate kinase to the cell nucleus. Restoration of three amino acids of nucleoplasmin sequence at either end of this sequence generated sequences that were able to locate pyruvate kinase to the cell nucleus. The 14-amino acid proposed minimal nuclear location sequence is present in the functional sequences, AVKRPAATKKAGQAKKK, RPAATKKAGQAKKKKLD, and the sequence AVKRPAATKKAGQAKKKKLD, which has additional amino acids at both ends. The minimal sequence element is therefore necessary but not sufficient for transport into the cell nucleus. This unusual feature of the nucleoplasmin nuclear location sequence suggests ways in which it could interact with the nuclear transport mechanism.