EXPRESSION OF CELL-SURFACE MARKERS AFTER HUMAN LYMPHOCYTE-B ACTIVATION
EXPRESSION OF CELL-SURFACE MARKERS AFTER HUMAN LYMPHOCYTE-B ACTIVATION
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DOI：
10.1073/pnas.78.6.3848
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
SCHLOSSMAN, SF
中科院分区：
文献类型：
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作者：
STASHENKO, P;NADLER, LM;SCHLOSSMAN, SF
研究了最近发现的2种人B淋巴细胞特异性抗原（B1和B2）在体内和体外B细胞活化后的命运。几乎所有正常淋巴结的B细胞中都存在B1和B2，大约50%的增生性淋巴结的B细胞中不存在B2。用商陆有丝分裂原刺激脾脏、扁桃体或外周血的B细胞，激活细胞在体外失去B2（4-5天），随后失去B1（6-7天）。在时间上，B2缺失伴随着表面IgD的缺失、T10的表达和胞浆内IgM的发生；B1丢失与表面IgG的获取和胞浆内IgG的出现相关。与来自次级淋巴器官的B细胞（B1++++B2++）相比，外周血B细胞通常只弱表达B2 (B1++++B2+)，在激活过程中B2消失之前，表现出短暂的B2表达增加到B1++++B2++表型。外周血可能含有相对不成熟的B细胞亚群。
The fate of 2 recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B cell activation in vivo and in vitro. Both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells from spleen, tonsil or peripheral blood were stimulated in vitro with pokeweed mitogen, activated cells lost B2 (days 4-5) and subsequently B1 (days 6-7). Temporally, B2 loss was accompanied by loss of surface IgD, expression of T10 and the development of intracytoplasmic IgM; B1 loss was correlated with the acquisition of surface IgG and the appearance of intracytoplasmic IgG. Peripheral blood B cells, on which B2 is normally only weakly expressed (B1++++B2+) in contrast to B cells from secondary lymphoid organs (B1++++B2++), exhibited a transitory increase in B2 expression to the B1++++B2++ phenotype prior to B2 disappearance during activation. Peripheral blood may contain a relative immature subpopulation of B cells.