Simultaneously promoting adhesion and osteogenic differentiation of bone marrow-derived mesenchymal cells by a functional electrospun scaffold
Simultaneously promoting adhesion and osteogenic differentiation of bone marrow-derived mesenchymal cells by a functional electrospun scaffold
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通过功能性电纺支架同时促进骨髓间充质细胞的粘附和成骨分化
DOI：
10.1016/j.colsurfb.2020.111040
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Zhentao Man
中科院分区：
文献类型：
--
作者：
Wei Li;Hailun Xu;Xiaojuan Han;Shui Sun;Qihao Chai;Xianxing Xu;Zhentao Man
静电纺丝技术是构建组织工程骨再生支架的常用技术。然而，纯静电纺丝支架不能富集种子细胞或促进其成骨分化。组织工程支架的生物功能化是目前的研究热点。因此，在这项研究中，骨髓间充质细胞（BM-MSC）特异性亲和肽E7和骨形态发生蛋白2（BMP-2）模拟肽伴随缀合到静电纺丝支架的表面上，以构建功能性PEB支架。PEB支架的表征表明，E7和BMP-2模拟肽成功地结合到静电纺丝支架的表面上。在生物学活性方面，PEB支架能够同步促进BM-MSC的粘附和成骨分化，这是由于E7和BMP-2模拟肽的共同递送，这被证明是上级的优于其他三种支架。因此，PEB支架为骨组织工程支架的构建提供了新的概念。
Electrospinning is a common technology to construct tissue engineering scaffolds for bone regeneration. However, pure electrospun scaffolds do not enrich seed cells or promote their osteogenic differentiation. Biological functionalization of tissue engineering scaffolds is currently a hot research topic. Therefore, in this study, the bone marrow-derived mesenchymal cells (BM-MSC)-specific affinity peptide E7 and a bone morphogenic protein 2 (BMP-2) mimetic peptide were concomitantly conjugated onto the surface of an electrospun scaffold to construct a functional PEB scaffold. Characterization of PEB scaffolds revealed that both E7 and BMP-2 mimetic peptides were successfully conjugated onto the surface of electrospun scaffolds. With regard to biological activity, the PEB scaffold could synchronously promote adhesion and osteogenic differentiation of BM-MSC as a result of the co-delivery of E7 and BMP-2 mimetic peptides, which proved superior compared with the other three scaffolds. Consequently, the PEB scaffold offers a new concept for the construction of bone tissue engineering scaffolds.