Anti-oxidative effects of D-allose, a rare sugar, on ischemia-reperfusion damage following focal cerebral ischemia in rat
Anti-oxidative effects of D-allose, a rare sugar, on ischemia-reperfusion damage following focal cerebral ischemia in rat
复制标题
DOI：
10.1016/j.neulet.2010.10.004
复制
发表时间：
2011-01-03
影响因子：
2.5
通讯作者：
Miyamoto, Osamu
中科院分区：
文献类型：
--
作者：
Nakamura, Takehiro;Tanaka, Shinji;Miyamoto, Osamu
本研究探讨D-阿洛糖对缺血性损伤的抗氧化作用。大鼠在戊巴比妥麻醉下短暂大脑中动脉闭塞（MCAO）1小时。在闭塞期间和再灌注后1小时静脉输注D-阿洛糖（400 mg/kg）。通过测量MCAO后7天的脑损伤（梗死和萎缩体积）和行为缺陷来检查D-阿洛糖对局灶性脑缺血的影响。在另一组大鼠中，在MCAO后24小时，研究了无嘌呤/无嘧啶脱碱基位点（AP位点）和8-羟基-2 '-脱氧鸟苷（8-OHdG）（氧化应激标志物），以检查D-阿洛糖的抗氧化作用。与赋形剂相比，D-阿洛糖给药可显著降低脑损伤和行为缺陷。D-阿洛糖还降低了AP位点的数量和8-OHdG水平。因此，本研究表明，D-阿洛糖具有抗氧化作用，并诱导局灶性脑缺血的神经保护作用。(C)2010爱思唯尔爱尔兰有限公司版权所有。
The present study investigates the anti-oxidative effects of D-allose on ischemic damage. Rats were subjected to transient middle cerebral artery occlusion (MCAO) for 1 h under pentobarbital anesthesia. D-allose was intravenously infused during occlusion and a further 1 h after reperfusion (400 mg/kg). The effects of D-allose on focal cerebral ischemia were examined by measuring brain damage (infarction and atrophy volume) and behavioral deficits 7 days after MCAO. In another set of rats, apurnic/apyrimidic abasic sites (AP-sites) and 8-hydroxy-2'-deoxyguanosine (8-OHdG), oxidative stress markers, were investigated 24h after MCAO to examine the anti-oxidative effects of D-allose. Brain damage and behavioral deficits were significantly decreased by D-allose administration compared to vehicle. The number of AP-sites and 8-OHdG levels were also reduced by D-allose. Thus, the present study suggests that D-allose has anti-oxidative effects and induces neuroprotection in focal cerebral ischemia. (C) 2010 Elsevier Ireland Ltd. All rights reserved.