Application of Dermal Microdialysis for the Determination of Bioavailability of Clobetasol Propionate Applied to the Skin of Human Subjects
Application of Dermal Microdialysis for the Determination of Bioavailability of Clobetasol Propionate Applied to the Skin of Human Subjects
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DOI：
10.1159/000330489
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Kanfer, I.
中科院分区：
文献类型：
--
作者：
Au, W. L.;Skinner, M. F.;Kanfer, I.
皮肤微透析用于评估局部皮质类固醇丙酸氯倍他索应用于人类受试者皮肤后的生物利用度。研究了丙酸氯倍他索4% m/v乙醇溶液对健康志愿者前臂4个部位的渗透作用。使用脂质乳状液Intralipid (R)作为灌注液，并在皮内指定位置插入具有2 kda截止的线性微透析探针。结果表明，脂内液可作为本研究目的亲脂性药物体内微透析的合适灌注液。此外，该研究清楚地表明，皮肤微透析是评估局部应用丙酸氯倍他索渗透皮肤的生物利用度/生物等效性的有价值的工具。巴塞尔S. Karger股份有限公司版权所有
Dermal microdialysis was used to assess the bioavailability of a topical corticosteroid, clobetasol propionate, following application onto the skin of human subjects. The penetration of clobetasol propionate from a 4% m/v ethanolic solution applied onto 4 sites on one forearm of healthy human volunteers was studied. A lipid emulsion, Intralipid (R), was used as the perfusate and linear microdialysis probes with a 2-kDa cutoff were inserted intradermally at the designated sites. The results indicated that Intralipid could be used as a suitable perfusate for in vivo microdialysis of this lipophilic drug of interest. Furthermore, the study clearly demonstrated the application of dermal microdialysis as a valuable tool to assess the bioavailability/bioequivalence of clobetasol propionate penetration into the skin following topical application. Copyright (C) 2011 S. Karger AG, Basel